Effectiveness of the Ethanol Extract Combination of Binahong and Patikan Kebo Against Staphylococcus aureus Causing Cellulitis
DOI:
https://doi.org/10.26740/lenterabio.v14n1.p113-118Keywords:
infection, secondary metabolites, Antibacterial, inhibition zoneAbstract
Cellulitis is a skin infection caused by Staphylococcus aureus. The use of antibiotics in treatment often has the potential to increase bacterial resistance. The secondary metabolites present in binahong and patikan kebo leaves have the potential to act as antibacterial agents. This study aimed to determine whether the combination of binahong and patikan kebo leaf extracts is more effective in inhibiting the growth of S. aureus compared to their single extracts. The antibacterial activity was tested using the disc diffusion method with negative control DMSO 10% and positive control amoxicillin 25 mcg. The concentrations of single extracts used were 40%, 50%, and 60%, while the combination extract test ratios were 1:3, 2:2, and 3:1. The data were analyzed using the Kolmogorov-Smirnov test and one-way ANOVA, followed by the Duncan test. The results of the antibacterial activity test showed that the combination of ethanol extracts of patikan kebo and binahong leaves had better effectiveness as an antibacterial against S. aureus compared to the single extract. The optimal combination of ethanol extracts of patikan kebo and binahong leaves in inhibiting the growth of S. aureus was in the ratio of 1:3, as it produced the largest inhibition zone with an average diameter of 1.11 ± 0.10 cm. Therefore, ethanol extracts combination of patikan kebo and binahong leaves can be used as the main ingredients for skin infection drugs.
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