The Effectiveness of Papaya Leaf Extract (Carica papaya L.) Against the In Vitro Bacterial Growth Inhibition of Staphylococcus aureus

Abstract

Mastitis caused by Staphylococcus aureus bacteria is often found in livestock. Papaya leaves contain antibacterial active compounds widely used as traditional medicine for various diseases. The purpose of this study was to determine the inhibition of papaya leaf extract (Carica papaya L.) against the growth of Staphylococcus aureus bacteria. Papaya leaf was extracted using maceration method with 96% ethanol. Inhibition test was performed using well-diffusion method. The concentrations of papaya leaf extract used were 15%, 30%, 45%, and 60%. The parameter of this research is the diameter of the inhibition zone for the growth of S. aureus bacteria. The diameter of the inhibition zone of S. aureus bacteria was analyzed using Duncan's test. The results of this study showed that papaya leaf extract at concentration of 30%, 45%, and 60% were able to inhibit the growth of S. aureus bacteria with average diameter of 7.667 mm, 11.333 mm, and 16.333 mm, but 15% concentration had not been able to inhibit the growth of
S. aureus bacteria. Based on the results of the study, it was shown that papaya leaf extract had antibacterial property, so further research was needed regarding the use of papaya leaf extract for alternative treatment of mastitis.