Effect of Longan Leaf Extract (Dimocarpus longan L.) on Total Cholesterol and Hepatic Histopathology in Type 2 Diabetes Mellitus Mice
DOI:
https://doi.org/10.26740/lenterabio.v14n3.p340-346Keywords:
cholesterol level, diabetes mellitus, hepatic histopathology, longan leafAbstract
Hypercholesterolemia occurring in type 2 diabetes mellitus can trigger chronic complications, one of which is liver damage. Longan leaves contain metabolites that function as antihyperlipidaemic agents and repair liver damage. This study used 24 male DDY mice divided into six groups: negative control (A), positive control (B), metformin (C), longan leaf extract at 28 mg/kg body weight (D), 42 mg/kg body weight (E), and 56 mg/kg body weight (F). Type 2 DM was induced orally with a high-fat diet (HFD) and alloxan administered intraperitoneally. Longan leaf extract was administered orally. Total cholesterol levels were measured using a cholesterol meter. Liver histological slides were prepared using paraffin methods and evaluated using a light microscope. Total cholesterol levels and liver histopathology were statistically significant. The results showed that the longan leaf extract significantly affected total cholesterol levels and liver histopathology (p<0.05). The 56 mg/kgBW dose of longan leaf extract yielded the best results for reducing total cholesterol levels by 17.25 and liver histopathology scores of 1.1. Therefore, longan leaf extract has the potential to be an antihypercholesterolemic agent and to improve liver damage.
References
Adhitama S, Kuswanti N and Khaleyla F, 2023. Pengaruh Ekstrak Daun Kedondong Terhadap Penurunan Kadar Kolesterol Total Dan Berat Badan Mencit Diabetes Melitus Tipe II. LenteraBio: Berkala Ilmiah Biologi 12 (3): 354-362.
Al Amin M, Najib A and Syahbana A, 2023. Efektivitas Ekstrak Daun Annona Muricata Terhadap Penurunan Kolesterol Total Pada Lansia Dengan Hiperkolesterolemia Di Panti Werdha Mojopahit Mojokerto. Professional Health Journal 5 (1): 80-89.
Ayunda RD and Malita S, 2024. Pemanfaatan Senyawa Flavonoid sebagai Antioksidan pada Penderita Hiperkolesterolemia: Studi Literatur. Jurnal Kedokteran (Unram Medical Journal) 13 (3): 177–187.
Dewi DS and Asman A, 2020. Hubungan Antara Kadar Glukosa Darah Dengan Derajat Ulkus Kaki Diabetik Pada Pasien Diabetes Mellitus. Journal Transformation of Mandalika 1(4): 203-205.
Decroli E, Nagara GK and Kam A, 2022. Mekanisme Molekuler dari Resistensi Insulin pada Diabetes Melitus Tipe Dua. Majalah Kedokteran Andalas, 45(4), 610-618.
El Qahar HA, 2020. Pengaruh Lidah Buaya Menurunkan Kadar Glukosa Darah Pada Diabetes Melitus Tipe 2. Jurnal Ilmiah Kesehatan Sandi Husada 9(2): 798-805.
Fahreza F, Hasni D, Vani AT and Jelmila SN, 2020. Gambaran Kadar Total Kolesterol Pada Pasien Prolanis Yang Mendapat Terapi Simvastatin Di Puskesmas Air Dingin 2018. Ibnu Sina: Jurnal Kedokteran dan Kesehatan-Fakultas Kedokteran Universitas Islam Sumatera Utara, 19(2), 53-62.
Fitriani RN, Sitasiwi AJ and Isdadiyanto S, 2020. Struktur hepar dan rasio bobot hepar terhadap bobot tubuh mencit (Mus musculus L.) jantan setelah pemberian ekstrak etanol daun mimba (Azadirachta indica A. Juss). Buletin Anatomi dan Fisiologi 5 (1): 75-83.
Gonfa YH, Bachheti A, Semwal P, Rai N, Singab AN and Bachheti RK, 2025. Hepatoprotective activity of medicinal plants, their phytochemistry, and safety concerns: A systematic review. Zeitschrift für Naturforschung C 80 (3-4): 61-73.
Guilherme A, Henriques F, Bedard AH and Czech MP, 2019. Molecular pathways linking adipose innervation to insulin action in obesity and diabetes mellitus. Nature Reviews Endocrinology 15 (4): 207–225.
Hendrawan S, Tamaro A, Angelina C and Firmansyah Y, 2023. Kegiatan Pengabdian Masyarakat dalam Rangka Peningkatan Kewaspadaan Masyarakat terhadap Penyakit Pre-Diabetes dan Diabetes Mellitus Tipe II dengan Edukasi dan Deteksi Dini Penyakit. Jurnal Pengabdian Ilmu Kesehatan 3(2): 36-49.
Hilma H, Della Putri NA and Lely N, 2021. Penentuan Kandungan Total Fenol Dan Total Flavonoid Ekstrak Daun Kelengkeng (Dimoncarpus longan Lour). Jurnal Ilmiah Farmako Bahari 12(1): 80-87.
International Diabetes Federation, 2021. IDF Diabetes Atlas(10th edition). International Diabetes Federation.
Kristófi R and Eriksson JW, 2021. Metformin as an anti-inflammatory agent: a short review. Journal of Endocrinology 251 (2): R11-R22.
Mahdavi A, Bagherniya M, Fakheran O, Reiner Ž, Xu S and Sahebkar A, 2020. Medicinal plants and bioactive natural compounds as inhibitors of HMG‐CoA reductase: A literature review. BioFactors 46 (6): 906-926.
Mahmudah R, Himaniarwati H and Imran SAA, 2024. Standarisasi dan Uji Aktivitas Antikolesterol Ekstrak Etanol Daun Labu Kuning (Cucurbita moschata Duch.) pada Mencit (Mus musculus). Journal Mandala Pharmacon Indonesia 10 (1): 111-121.
Meidayanti D, 2021. Benefits of Lycopene in Tomatoes as a Prevention Against Atherosclerosis. Jurnal Medika Hutama 2 (03 April): 906-910.
Murray RK, Granner DK, Mayes PA and Rodwell VW, 2003. Harper's Illustrated Biochemistry. 26th ed. New York: McGraw-Hill Medical.
Oktaviona EEP, Qomariyah N and Khaleyla F, 2023. Aktivitas Hepatoprotektif Ekstrak Daun Phyllanthus Acidus L. Pada Mencit Diabetes Mellitus Tipe 2. LenteraBio: Berkala Ilmiah Biologi 12 (3): 381-388.
Odoma S, Twinomugisha F, Neeza T and Funso-Babarimisa F, 2025. Evaluation of the anthelmintic activity of ethanol leaf extract of Dimocarpus longan (Lour.) against Taenia solium. Journal of Pharmacy & Bioresources 22 (1): 45-52.
Pane M, Rahman A and Ayudia E, 2021. Gambaran penggunaan obat herbal pada masyarakat indonesia dan interaksinya terhadap obat konvensional tahun 2020. Journal of Medical Studies 1 (1): 40-62.
Pangkahila E, Linawati NM, Sugiritama IW and Siswanto FM, 2019. Pelatihan Fisik Berlebih Meningkatkan Indeks Apoptosis pada Hepatosit Tikus (Rattus norvegicus) Wistar Jantan. Jurnal Biomedik: JBM 11 (3).
Pasaribu SF, Wiboworini B and Kartikasari LR, 2021. Effect of Germinated Black Rice Krisna Extract on Fasting Blood Glucose and Body Weight in Diabetes Mellitus Rats. International Journal of Nutrition Sciences 6 (4): 194-200.
PERKENI, 2021. Pedoman Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 Dewasa di Indonesia. Jakarta: PB PERKENI.
Rusdi MS, 2020. Hipoglikemia Pada Pasien Diabetes Melitus. Journal Syifa Sciences and Clinical Research (JSSCR) 2(2): 83-90.
Salamah N and Widyasari E, 2015. Aktivitas antioksidan ekstrak metanol daun kelengkeng (Euphoria longan (L) Steud.) dengan metode penangkapan radikal 2, 2’-difenil-1-pikrilhidrazil. Pharmaciana 5 (1): 25–34.
Setiadi E, Peniati E and Susanti RSR, 2020. Pengaruh Ekstrak Kulit Lidah Buaya Terhadap Kadar Gula Darah Dan Gambaran Histopatologi Pankreas Tikus Yang Diinduksi Aloksan. Life Science 9(2): 171-185.
Soetrisno S, Arinekso B and Sulistyowati S, 2019. The effect of L-arginine therapy on hepatocyte cell damage in the mice (Mus musculus) model of preeclampsia. Journal of the Medical Sciences (Berkala Ilmu Kedokteran) 51 (4): 316-324.
Susanti L, Widodo S, Bahri S and Indriasari W, 2016. Uji Formulasi Sediaan Salep Ekstrak Batang Brotowali (Tinospora crispa L. Miers) Kombinasi Zeolit Terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa. Jurnal Kelitbangan 4 (03): 234–243.
Syari FR, Hendrianingtyas M and Retnoningrum D, 2019. Hubungan Lingkar Pinggang Dan Visceral Fat Dengan Kadar Ferritin Serum Pada Obesitas. Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal) 8 (2): 701-712.
van Gerwen J, Shun-Shion AS and Fazakerley DJ, 2023. Insulin signalling and GLUT4 trafficking in insulin resistance. Biochemical Society Transactions 51 (3): 1057–1069.
Widiasari KR, Wijaya IMK and Suputra PA, 2021. Diabetes Melitus Tipe 2: Faktor Risiko, Diagnosis, Dan Tatalaksana. Ganesha Medicina 1(2): 114-120.
Wu J, Lv S, Zhao L, Gao T, Yu C, Hu J and Ma F, 2023. Advances in the study of the function and mechanism of the action of flavonoids in plants under environmental stresses. Planta 257 (6): 108.
Yuningrum H, Rahmuniyati ME and Lende TDP, 2022. Konsumsi Gorengan dan Asupan Kolesterol Berhubungan dengan Kejadian Hiperkolesterolemia pada Mahasiswa. Jurnal Kesmas (Kesehatan Masyarakat) Khatulistiwa 9 (2): 98-108.
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