Effect of Red Turi Leaf (Sesbania grandiflora L.) Extract on The Length of Ulcers and Leukocyte Count in Diabetic Mice

Abstract

Hyperglycemia in diabetes mellitus interferes with  ulcer healing process and the body's immune response. Red turi leaf contains secondary metabolites with potential to promote healing of diabetic ulcers. The purpose of this study was to determine the effect of ethanolic extract of red turi leaf (Sesbania grandiflora L.) on ulcer length and leukocyte counts in alloxan-induced mice. This study was an experimental research using a completely randomized design (CRD) with 24 male mice which were divided into six treatment groups, negative control (KN), positive control (KP), Glibenclamid (GLB),  DII (250 mg/kgBW), DII (400 mg/kg), DIII (500 mg/kgBW). Mice were induced diabetes melitus by injected alloxan 130 mg/kgBW intraperitoneally. Ethanolic extract of red turi leaf (Sesbania grandiflora L.) was administered orally for 14 days. Ulcer lengths were measured on the H1, H4, H6, H7, H10 and H14. Leukocytes were counted on the 15^th day (after treatment). Data were analyzed statistically.. The results showed that red turi leaf extract had an effect on ulcer length (p<0,05) and leukocytes count (p<0,05). The optimal dose for diabetic ulcers was for DII (400 mg/kgBW) while the optimal dose for leukocyte count was for DI (250 mg/kgBW). Ethanolic extract of red turi leaves could be concluded to have potential for ulcer healing agent and able to maintains leukocytes count of diabetic mice.