Effects of Adding Glutathione to CEP Diluent on Post-Thawing Sperm Quality of Bali Cattle
DOI:
https://doi.org/10.26740/lenterabio.v14n1.p63-71Keywords:
antioxidant, freezing semen, genetic diversity and farmed animals, sperm qualityAbstract
Storing spermatozoa at freezing temperatures can expose spermatozoa cells to reactive oxygen species (ROS) and damage their quality. Therefore, the spermatozoa require storage in certain diluents treated with antioxidants. This study aims to determine the effect of adding glutathione as an antioxidant in CEP diluent on the quality of frozen Bali cattle semen. This study used a Completely Randomized Design with six doses of glutathione (0, 0.5, 0.75, 1, 1.25, and 1.5 mM) in CEP diluent with four repetitions. Fresh semen obtained from Balinese cows is approximately six years old and collected using the Artificial Vagina method. The ANOVA test showed that adding various doses of glutathione significantly affected the quality of post-thawing spermatozoa (P<0.05). Duncan's test showed that 0.75 mM glutathione had the highest post-thawing for all parameters, motility (55.30 ± 2.95%), viability (71.26 ± 2.23%), and membrane integrity (58.08±2.52%). This study concluded that adding 0.75 mM glutathione was the best dose to maintain the quality of Bali cattle spermatozoa post-thawing.
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