Effect of Bruguiera gymnorrhiza Leaf Extract on malondialdehyde (MDA) Levels and Pancreatic Histopathology in Diabetic Mice
DOI:
https://doi.org/10.26740/lenterabio.v13n2.p244-252Keywords:
Diabetes, Health care, Immunology, Universal HealthAbstract
Diabetes mellitus causes an increase in malondialdehyde levels and pancreatic ? damage in the body. Mangrove leaf extract (Bruguiera gymnorrhiza) has bioactive compounds to decrease MDA levels and repair pancreatic ? cells. This study aimed to determine the effect of Bruguiera gymnorrhiza leaf extract on malondialdehyde levels and pancreatic ? cells. This study used 24 mice divided into 6 groups: control group, positive control (alloxan induction and glibenclamide treatment), negative control (alloxan induction without treatment), 200 mg/kgBB dose, 400 mg/kgBB dose, and 600 mg/kgBB dose group. MDA levels in mice serum were measured using the TBARS method, and pancreatic ?-cells were analyzed using the scoring method. Data analysis used descriptive analysis for MDA levels of mice, the Kruskall-Wallis, and the Mann-Whitney tests used for scoring data analysis of pancreatic ?-cell damage. The results showed that a dose of 400 mg/kgBB was the best dose to reduce MDA levels, and a dose of 600 mg/kgBB was the best in repairing pancreatic ? cells. The conclusion of this study is that mangrove leaf extract (Bruguiera gymnorrhiza) has an effect in reducing MDA levels and repairing pancreatic ? cells in diabetic mice.
References
Chandra K, Singh P, Dwivedi S and Jain S, 2019. Diabetes Mellitus and Oxidative Stress: A Co-relative and Therapeutic Approach. Journal of Clinical and Diagnostic Research Vol 13 (5): 7-12.
Decroli E, 2019. Diabetes Melitus Tipe 2. Padang: Pusat Penerbitan Bagian Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Andalas.
Dewatisari WF, 2020. Perbandingan Pelarut Kloroform dan Etanol terhadap Rendemen Ekstrak Daun Lidah Mertua (Sansevieria trifasciata. Prain) menggunakan Metode Maserasi. Prosiding Seminar Nasional Biologi Vol 6 (1): 127-132.
Dheer R and Bhatnagar P, 2010. A Study of the Antidiabetic Activity of Barleria prionitis Linn. Indian Journal of Pharmacology Vol 42 (2): 70-73.
Fang JY, Lin CH, Huang TH and Chuang SY, 2019. In Vivo Rodent Models of Type 2 Diabetes and Their Usefulness for Evaluating Flavonoid Bioactivity. Nutrients Vol 11 (3): 530.
Ghorbani A, Rashidi R and Shafiee-Nick R, 2019. Flavonoids for Preserving Pancreatic Beta Cell Survival and Function: a Mechanistic Review. Biomedicine & Pharmacotherapy Vol 111: 947-957.
Infodatin, 2014. Situasi dan Analisis Diabetes, Jakarta: Pusat Data dan Informasi Kementerian Kesehatan RI.
International Diabetes Federation (IDF), 2021. IDF Diabetes Atlas. Abu Dhabi: International Diabetes Federation.
Jung CH and Choi KM, 2017. Impact of High-Carbohydrate Diet on Metabolic Parameters in Patients with Type 2 Diabetes. Journal Nutrients Vol 9 (4): 1-21.
Kawatu C, Bodhi W, and Mongi J, 2013. Uji Efek Ekstrak Etanol Daun Kucing-Kucingan (Acalypha indica L.) terhadap Kadar Gula Darah Tikus Putih Jantan Galur Wistar (Rattus novergicus), Jurnal Ilmiah Farmasi Vol 2 (1): 81-87.
Marjani A, 2010. Lipid Peroxidation Alterations in Type 2 Diaroraabetic Patients. Pak J Biol Sci Vol 13 (15): 723-730.
Maulidiyanti ETS, 2017. Perbedaan Hasil Pemeriksaan Kadar Glukosa Darah 2 Jam PP dengan menggunakan Glukometer dan Analyzer pada Penderita Diabetes Melitus. The Journal of Muhammadiyah Medical Laboratory Technologist Vol 1(1): 16.
Mustofa MS, Purwaningsih E dan Pendrianto P, 2022. Pengaruh Ekstrak Daun Kenikir Terhadap Peningkatan Diameter Pulau Langerhans Tikus Model Diabetes Melitus Tipe 2. Journal of Current Pharmaceutical Sciences Vol 5 (2): 471-477.
Newsholme P, Keane KN, Carlessi R and Cruzat V, 2019. Oxidative Stress Pathways in Pancreatic ?-cells and Insulin-Sensitive Cells and Tissues: Importance to Cell Metabolism, Function, and Dysfunction. American Journal of Physiology-Cell Physiology Vol 317 (3): C420-C433.
Nopiyanti HT, Agustriani F, Isnaini I, and Melki M, 2016, Skrining Nypa Fruticans sebagai Antibakteri Bacillus Subtilis, Escherichia Coli dan Staphylococcus Aureus. Jurnal Maspari Vol 8(2): 83-90.
Rahayu N, 2015. Profil Malondialdehyde dan Kolesterol Darah Ayam Petelur Fase Layer pada Temperature Humidity Index yang Berbeda. Students e-Journal Vol 4 (1): 1-11.
Suastuti NIGAM, Dwi AIGA, Kunti SPD, dan Ni Komang Ariati, 2015. Pemberian Ekstrak Daun sirsak (Annona muricuta) untuk Memperbaiki Kerusakan Sel Beta Pankreas melalui Penurunan Kadar Glukosa Darah, Advanced Glycation and Product dan 8-Hidroksi-2-Dioksiguanosin pada Tikus Wistar Hiperglikemia. Jurnal Kimia Jurusan Kimia FMIPA Universitas Udayana Vol 9 (2): 289-295.
Tandi J, Rizky M, Mariani R dan Alan F, 2017. Uji Efek Ekstrak Etanol Daun Sukun (Artocarpus altilis (Parkinson Ex F. A. Zorn) terhadap Penurunan Kadar Glukosa Darah, Kolesterol Total dan Gambaran Histopatologi Pankreas Tikus Putih Jantan (Rattus norvegicus) Hiperkolesterolemia Diabetes. Jurnal Sains dan Kesehatan Vol 1 (8): 384-396.
Tangvarasittichai S, 2015. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World Journal of Diabetes Vol 6(3): 456-480.
Yanti ED, Dewi NWS dan Jawi IM, 2019. Kombinasi Ekstrak Sambiloto dengan Metformin Lebih Baik dalam Memperbaiki Sel Beta Pulau Langerhans dari pada Metformin Tunggal pada Tikus Diabetes. E-Jurnal Medika Vol 8 (2): 1-5.
Zaetun S, Dewi LBK, Wiadnya IBR dan Gede LS, 2019. Profil Kadar MDA (Malondialdehyde) Sebagai Penanda Kerusakan Seluler Akibat Radikal Bebas pada Tikus yang Diberikan Air Beroksigen. Jurnal Analis Medika Biosains (JAMBS) Vol 4 (2): 63-68.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 LenteraBio : Berkala Ilmiah Biologi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Hak Cipta (c) LenteraBio: Berkala Ilmiah Biologi
Karya ini dilisensikan di bawah Lisensi Internasional Creative Commons Attribution-NonCommercial 4.0.
Pemberitahuan Hak Cipta.
Hak cipta dari artikel yang diterima untuk diterbitkan akan diberikan kepada jurnal sebagai penerbit jurnal. Hak cipta yang dimaksud meliputi hak untuk menerbitkan artikel dalam berbagai bentuk (termasuk cetak ulang). Jurnal mempertahankan hak penerbitan atas artikel yang diterbitkan.
Abstract views: 202
,
PDF Downloads: 355
