Pengaruh Ekstrak Daun Bruguiera gymnorrhiza terhadap Kadar Malondialdehid (MDA) dan Gambaran Histopatologi Pankreas pada Mencit Diabetes

Jannatul Makwa; Erlix Rakhmad Purnama

doi:10.26740/lenterabio.v13n2.p244-252

Authors

Jannatul Makwa Universitas Negeri Surabaya
Erlix Rakhmad Purnama

DOI:

https://doi.org/10.26740/lenterabio.v13n2.p244-252

Keywords:

Diabetes, Health care, Immunology, Universal Health

Abstract

Diabetes mellitus causes an increase in malondialdehyde levels and pancreatic β damage in the body. Mangrove leaf extract (Bruguiera gymnorrhiza) has bioactive compounds to decrease MDA levels and repair pancreatic β cells. This study aimed to determine the effect of Bruguiera gymnorrhiza leaf extract on malondialdehyde levels and pancreatic β cells. This study used 24 mice divided into 6 groups: control group, positive control (alloxan induction and glibenclamide treatment), negative control (alloxan induction without treatment), 200 mg/kgBB dose, 400 mg/kgBB dose, and 600 mg/kgBB dose group. MDA levels in mice serum were measured using the TBARS method, and pancreatic β-cells were analyzed using the scoring method. Data analysis used descriptive analysis for MDA levels of mice, the Kruskall-Wallis, and the Mann-Whitney tests used for scoring data analysis of pancreatic β-cell damage. The results showed that a dose of 400 mg/kgBB was the best dose to reduce MDA levels, and a dose of 600 mg/kgBB was the best in repairing pancreatic β cells. The conclusion of this study is that mangrove leaf extract (Bruguiera gymnorrhiza) has an effect in reducing MDA levels and repairing pancreatic β cells in diabetic mice.

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