Aktivitas Hepatoprotektif Ekstrak Daun Phyllanthus acidus L. pada Mencit Diabetes Mellitus Tipe 2

Erin Eka Putri Oktaviona; Nur Qomariyah; Firas Khaleyla

doi:10.26740/lenterabio.v12n3.p381-388

Authors

Erin Eka Putri Oktaviona Universitas Negeri Surabaya
Nur Qomariyah State University of Surabaya
Firas Khaleyla State University of Surabaya

DOI:

https://doi.org/10.26740/lenterabio.v12n3.p381-388

Keywords:

Diabetes, Liver, Phyllanthus acidus, obesity

Abstract

In patients with hyperglycemia and hypercholesterolemia, oxidative stress that causes hyperinsulinemia resulting in the formation of free radicals that can trigger inflammation and cell death in the liver. Reactive oxygen species (ROS) are free radicals that can damage tissues and are captured or neutralized by flavonoid compounds in P. acidus leaf that act as antioxidants. This study aims to examine the effect of P. acidus leaf extract in liver histopatology and Hepatosomatic Index (HSI) in a type 2 diabetes mellitus mice model. In this study, 24 male Deutsch Denken Yoken (DDY) strain mice were used, and divided into six treatment groups, namely the control group, the negative control group, the positive group, a dose of 215 mg/kg BW, a dose of 230 mg/kg BW, and a dose of 245 mg/kg BW. Data analysis with statistical tests. Liver histology scoring was calculated using the Kruskal-Wallis test (p<0.05) followed by the Mann-Whitney test. A one-way ANOVA test was performed for HSI data results (p<0.05) and resumed with Duncan's test. The results of the research on the level of liver damage showed there was an effect of giving P. acidus leaf extract on the level of liver damage (p<0.05). In addition, the administration of P. acidus extract also affects the HSI value (p<0.05). Based on the results, it can be concluded that the dose 215 mg/kg BW of P. acidus leaf extract affected liver damage and Hepatosomatic Index (HSI).

References

Andreas H, Trianto HF, Ilmiawan MI, 2015. Gambaran Histologi Regenerasi Hati Pasca Penghentian Pajanan Monosodium Glutamat pada Tikus Wistar. EJournal Kedokteran Indonesia, 3(1): 29-36.

Armansyah TRT, Sutriana A, Aliza D, Vanda H, Rahmi E, 2010. Aktivitas Hepatoprotektif Ekstrak Etanol Daun Kucing-Kucingan (Acalypha indica L.) pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol. Jurnal Ilmiah Ilmu-Ilmu Peternakan, XIII(6): 292-298.

Ashwini L, Benakappa S, Anjanayappa HN, Akshay L, 2016. Observation on the Gonado-Somatic Index-GSI and Hepato-Somatic Index-HSI of Decapterus russelli Mangaluru coast. International Journal of Engineering Science ang Computing, 6(6): 7396-7399.

Fakhrurrazi, Hakim RF, Chairunissa A, 2020. Efek Ekstrak Daun Ceremai (Phyllanthus acidus (L.) Skeels) Terhadap Penyembuhan Luka Mukosa Tikus Wistar (Rattus norvegicus). Cakrodonya Dental Jurnal. 12(2): 119-125.

Gumantara MPB, Oktarlina RZ, 2017. Perbandingan Monoterapi dan Kombinasi Terapi Sulfonilurea-Metformin terhadap Pasien Diabetes Melitus Tipe 2. Majority, 6(1): 55-56.

Jain NK, dan Singhai AK, 2011. Protective Effect Phyllanthus acidus (L.) Skeels Leaf Extracts on Acetaminophen and Thioacetamide Induce Hepatic Injuries in Wistar Rats. Asian Pacific Journal of Tropical Medicine, 470-474.

Khaleyla F, Ducha N, Bashri A, 2021. Metode Parafin untuk Sediaan Irisan Jaringan Hewan. Surabaya: Jurusan Biologi UNESA.

Ma’ruf A, Hardhana B, Widiantini W, Pangribowo S, Mulya D, 2020. Tetap Produktif, Cegah, dan Atasi Diabetes Mellitus. Jakarta: P2PTM Kementerian Kesehatan RI.

Mohamed J, Nafizah AHN, Zariyanti AH, Budin SB, 2016. Mechanisms of Diabetes-Induced Liver Damage. SQU Medical Journal, 16(2):132-141.

Mordue DG, Monroy F, Regina ML, Dinarello CA, Sibley LD, 2001. Acute Toxoplasmosis Leads to Lethal Overproduction of Th1 Cytokines. Jurnal of Immunology, 167:4574-4584.

Narulita E, Iqbal M, Surakhman G, 2019. A Novel Agent of Myrmeleon formicarius Ektract for Diabetic Ulcer Infection. Indonesian Journal of Biotechnology and Biodiversity, 3(2): 48-54.

Navarro V, Khan I, Björnsson E, Seeff LB, Serrano J, Hoofnagle JH, 2017. Liver Injury from Herbal and Dietary Supplements. Hepatology, 65(1): 363-373.

Nazarudin Z, Muhimmah I, Fidianingsih I, 2017. Segmentasi Citra untuk Menentukan Skor Kerusakan Hati secara Histologi. Seminar Nasional Informatika Media, VIII: 15-21.

Niendya A, Djaelani MA, Suprihatin, 2011. Rasio Bobot Hepar-Tubuh Mencit (Mus musculus L.) setelah Pemberian Diezepam, Formalin, dan Minuman Beralkohol. Buletin Anatomi dan Fisiologi, 19(1): 16-27.

Prameswari OM, Widjanarko SM, 2014. Uji Efek Ekstrak Air Daun Pandan Wangi Terhadap Penurunan Kadar Glukosa Darah dan Histopatologi Tikus Diabetes Mellitus. Jurnal Pangan dan Agroindustri, 2(2): 16-27.

Putri RA, Sitasiwi AJ, Kasiyati, 2021a. Pengaruh Paparan Ekstrak Biji Klabet (Trigonella foenum-graecum L.) terhadap Kadar Hemoglobin, Indeks Hepatosomatik dan Kadar Protein Hepar Tikus Putih (Rattus norvegicus L.). Jurnal Pro-Life, 8(3): 218-226.

Putri WCW, Yuliawati, Rahman H, 2021b. Uji Aktivitas Hepatoprotektor Ekstrak Etanol Daun Rambutan (Naphelium lappaceum L.) pada Mencit Putih Jantan Yang Diinduksi Parasetamol. Jurnal Farmasi Indonesia, 18(2): 148-156.

Ratri PR, Yulianti A, Restuti ANS, 2021. Pengaruh Pemberian Minuman Coklat (Theobroma cacao L.) Terhadap Berat Basah Organ Hati Tikus Diabetes Mellitus. Jurnal Ilmu Kesehatan, 2(3): 74-79.

Rena G, Hardie DG, Pearson ER, 2017. The Mechanism of Action of Metformin. Diabetologia, 60: 1577-1585.

Rias YA, Sutikno E, 2017. Hubungan Antara Berat Badan dengan Kadar Gula Darah Acak pada Tikus Diabetes Mellitus. Jurnal Wiyata, 4(1): 72-77.

Riwu M, Subarnas A, Lestari K, 2015. Korelasi Faktor Usia, Cara Minum, dan Dosis Obat Metformin terhadap Risiko Efek Samping pada Penderita Diabetes Melitus Tipe 2. Jurnal Farmasi Klinik Indonesia, 4(3): 151-161.

Sari LM, 2018. Apoptosis: Mekanisme Molekuler Kematian Sel. Cakradonya Dental Journal, 10(2): 65-70.

Setiadi E, Peniati E, Susanti R, 2020. Pengaruh Ekstrak Kulit Lidah Buaya Terhadap Kadar Gula Darah dan Gambaran Histopatologi Pankreas Tikus yang Diinduksi Aloksan. Life Science, 9(2): 171-185.

Sijid StA, Muthiadin C, Zulkarnain, Hidayat ArS, Amelia RR, 2020. Pengaruh Pemberian Tuak Terhadap Gambaran Histopatologi Hati Mencit (Mus musculus) ICR Jantan. Jurnal Pendidikan Matematika dan IPA, 11(2):193-205.

Sitasiwi AJ, Isdadiyanto S, Mardiati SM, 2018. Effect of Ethanolic Neem (Azadirachta indica) leaf extract as an herb contraceptive on Hepato-somatic Index of the male mice (Mus musculus). Journal of Physics. doi: 10.1088/1742-6596/1025/1/012043.

Soelistijo SA, Suastika K, Lindarto D, Decroli E, Permana H, Sucipto KW, Kusnadi Y, Budiman, Ikhsan R, Sasiarini L, Sanusi H, Nugroho N, Susanto H, 2021. Pedoman Pengelolaan dan Pencegahan Diabetes Mellitus Tipe 2 Dewasa Di Indonesia 2021. PB PERKENI.

Sunaryo H, Rahmania RA, Dwitiyanti, Siska, 2015. Aktivitas Antioksidan Kombinasi Ekstrak Jahe Gajah (Zingiber officinale Rosc.) dan Zink Berdasarkan Pengukuran MDA, SOD dan Katalase pada Mencit Hiperkolesterolemia dan Hiperglikemia dengan Penginduksi Streptozotosin. Jurnal Ilmu Kefarmasian Indonesia, 13(2): 187-193.

Sundaram R, Naresh R, Shanthi P, Sachdanandam P, 2013. Modulatory Effect of Green Tea Extract on Hepatic Key Enzymes of Glucose Metabolism in Streptozotocin and High Fat Diet Induced Diabetic Rats. Phytomedicine, 1-8.

Tatto D, Dewi NP, Tibe F, 2017. Efek Antihiperkolesterol dan Antihiperglikemik Ekstrak Daun Ceremai (Phyllanthus acidus (L.) Skeels) pada Tikus Putih Jantan (Rattus norvegicus) Hiperkolesterol Diabetes. Jurnal Farmasi Gelenika, 3(2): 157-164.

Tram NCT, Son NT, Nga NT, Phuong VTT, Cuc NT, Phuong DT, Truan G, Cuong NM, Thao DT, 2017. The Hepatoprotective Activity of a New Derivative Kaempferol Glycoside from the Leaves of Vietnamese Phyllanthus acidus (L.) Skeels. Medicinal Chemistry Research, 26: 2057-2064.

Treuting PM, Dintzis S, Montine KS, 2017. Comparative Anatomy and Histology: a Mouse, Rat, and Human Atlas. Academic Press.

Wahyuningtyas P, Sitasiwi AJ, Mardiati SM, 2018. Hepatosomatic Index (HSI) dan Diameter Hepatosit Mencit (Mus musculus) Setelah Paparan Ekstrak Air Biji Pepaya (Carica papaya L.). Jurnal Biologi, 7(1): 1-10.

Yuneldi RF, Saraswati TR, Yuniwarti EYW, 2018. Profile of SGPT and SGOT on Male Rats (Rattus norvegicus) Hyperglycemic After Giving Insulin Leaf Extract (Tithonia diversifolia). Biosaintifika, 10(3): 519-525.

Zhang Y, Dong H, Wang M, Zhang J, 2016. Quercation Isolated from Toona sinesis Leaves Attenuates Hyperglycemia and Protect Hepatocytes in High-Carbohydrate/High-Fat Diet and Alloxan Induced Experimental Diabetic Mice. Journal of Diabetes Research, 8492780. http://dx.doi.org/10.1155/2016/8492780.