Antihyperglycemic Effect of Ceremai Leaf Extract (Phyllanthus acidus) in Mice (Mus musculus) with Diabetes Mellitus Type II
DOI:
https://doi.org/10.26740/lenterabio.v12n3.p363-370Keywords:
Diabetes, gula darah puasa, high fat diet, pankreas, Phyllanthus acidusAbstract
Induction of High Fat Diet and alloxan causes increasing free fatty acids that trigger the formation of reactive oxygen species and insulin resistance then the metabolism of blood sugar levels is disrupted. The antioxidant of Phyllanthus acidus leaf extract has the potential to stabilize insulin. This study aimed to determine the effect of P. acidus leaf extract on reducing fasting blood glucose (FBG) and pancreatic histopathology of type II diabetes mellitus (DM) mice. The experimental study used 24 DDY strain male mice, divided into six groups consisting of the control group, negative control (HFD and Alloxan), positive (Metformin), dose I (215 mg/kg BW), dose II ( 230 mg/kg BW), and dose III (245 mg/kg BW). HFD induction was conducted for 8 weeks combined with a single dose of alloxan and 21 days of extract. Data were analyzed with One-way ANOVA, continuing with Duncan's test. The results proved that this extract significantly affected decreasing FBG (p=0.004) and level of pancreatic damage (p=0.008). Based on results, it can be concluded that P. acidus leaf extract has an antihyperglycemic effect on reducing FBG of type II DM mice within normal also being able to trigger pancreatic β cell regeneration compared to other treatments with dose III (245 mg/kg BW) as the optimal extract dose.
References
Abdurrachim R dan Annisa RD, 2018. Fiber Intake and Physical Excercise Contributed to Blood Glucose Level in Outpatients With Type 2 Diabetes Mellitus. Jurnal Gizi Dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics); 5(2): 66.
Adichandra B dan Gunardi G, 2014. Pengaruh Pemberian Kayu Manis ( Cinnamomun burmannii ) Terhadap Kadar Glukosa Darah Tikus Wistar Jantan Yang Diberi Beban Glukosa. Semarang: Fakultas Kedokteran Universitas Diponegoro.
Aisyah K, 2021. Identifikasi Badan Keton Pada Urine Penderita Diabetes Mellitus Tipe 2 Systematic Review. Medan: Poltekkes Kemenkes Medan.
Akrom HP, Harjanti PD, dan Armansyah T, 2014. Efek Hipoglikemik Ekstrak Etanol Umbi KetelaRambat (Ipomoea batatas P) (eeukr) Pada Mencit Swiss Yang Diinduksi Aloksan. Pharmaçiana; 4(1): 65-76.
Anwar K, Fadlillaturrahmah F, dan Sari DP, 2017, Analisis Kandungan Flavonoid Total Ekstrak Etanol Daun Binjai (Mangiferacaesia jack .) dan Pengaruhnya Terhadap Kadar Glukosa Darah Tikus Yang Diinduksi Fruktosa-Lemak Tinggi. Jurnal Ilmiah Ibnu Sina; 2(1): 20–30.
Dusaulcy R, Handgraaf S, Visentin F, Howald C, Dermitzakis ET, Philippe J, dan Gosmain Y, 2019. High-Fat Diet Impacts More Changes in Beta-Cell Compared to Alpha-Cell Transcriptome. PLoS ONE; 14(3).
Erni AM dan Faridah A, 2014. Pengaruh Pemberian Minyak Mandar yang Ditambahkan Bubuk Daun Sukun (Arthocarpus altilis) terhadap Kadar Kolesterol Mencit (Mus musculus). Jurnal Bionature; 15(2).
Etuk EU, 2010. Animals Models for Studying Diabetes Melitus. Agric Biol J N Am; 1(2): 130-134.
Fahrumnisa AR, 2022. Penatalaksanaan Holistik Pada Laki Laki Usia 58 Tahun Dengan Diabetes Melitus Tipe 2 Melalui Pendekatan Kedokteran Keluarga. Jurnal Penelitian Perawat Profesional; 4(1): 61–70.
Fatimah RN, 2015. Diabetes Melitus Tipe 2. Jurnal Majority; 4(5): 93–101.
Firdaus F, Rimbawan R, Marliyati SA, dan Roosita K, 2016. Model Tikus Diabetes Yang Diinduksi Streptozotocin-Sukrosa Untuk Pendekatan Penelitian Diabetes Melitus Gestasional Streptozotocin, Sucrose-Induce Diabetic Male Rats Model for Research Approach of Gestational Diabetes Mellitus. PERENNIAL; 12(1): 29–34.
Ghulaxe C dan Verma R, 2015. A Review on Transdermal Drug Delivery Systems. The Pharma Innovation Journal; 4(1): 37–43.
Hartono RI dan Simanjutak K, 2022. Efektivitas Pemberian Suplemen Omega-3 terhadap Kadar Kolesterol Total Pada Tikus Galur Wistas (Rattus norvergicus) Yang Diinduksi Aloksan. Jurnal Kedokteran Syiah Kuala; 2(3).
Hasibuan R, Siregar SU, Nazliah R, Julyanti E, Simamora SS, dan Hasibuan LR, 2021. Pengaruh Ekstrak Daun Haramonting (Rhodomytus tomentosa) Sebagai Antidiabetes Terhadap Berat Badan dan Kondisi Histologi Penkreas Mencit (Mus muscullus L.). Jurnal Education and Development; 9(2).
Khaleyla F, Ducha N, dan Bashri A, 2021. Metode Parafin untuk Sediaan Irisan Jaringan Hewan. Surabaya: Universitas Negeri Surabaya.
Lei X dan Vatamaniuk M, 2011. Two Tales of Antioxidant Enzymes on β Cells and Diabetes. Antioxidants & Redox Signaling; 14(3): 489-503.
Madihah M, Alfina F, dan Gani YY, 2016. Kadar Glukosa Darah dan Gambaran Histologis Pankreas Mencit (Mus musculus L.) yang Diinduksi Aloksan Setelah Perlakuan Ekstrak Rimpang Temu Mangga (Curcuma mangga Val.). Jurnal Biologi Udayana; 20(2): 64-48.
Ningrum NFS dan Wahyuni AS, 2021. Pengaruh Pemberian Ekstrak Etanol 50 % Daun Matoa (Pometia pinnata J . R . Forst & G . Forst ) Terhadap Kadar Glikogen Hati Tikus. In Prosiding University Research Colloqium; 147–154.
Nubatonis DC, Ndaong NA, dan Selan YN, 2015. Pengaruh Pemberian Ekstrak Etanol Daun Sambiloto (Andrographis paniculata Nees) Terhadap Histopatologi Pankreas Mencit (Mus musculus) Diabetes Melitus (DM) Tipe I. Jurnal Kajian Veteriner; 3(1): 31-40.
Nurhadiyansah N, Dewi MK, dan Kharisma Y, 2019. Zingiber officinale Roscae Water Fraction Effect to Blood Glucose in Mice with High Lipid Die. Prosiding Pendidikan Dokter; 5(1): 640–650.
Rahmania FL, 2020. Perbandingan Tingkat Kerusakan dan Diameter Pulau Langerhans Pankreas Tikus Diabetes Melitus Dengan Pemberian Ekstrak Daun Binahong dan Sambiloto. Jakarta: Universitas Islam Negeri Syarif Hidayatullah Jakarta.
Ratnaningtyas NI, Hernayanti H, Andarwanti S, Ekowati N, Purwanti ES, dan Sukmawati D, 2018. Effects of Ganoderma lucidum Extract on Diabetic Rats. Biosaintifika: Journal of Biology & Biology Education; 10(708): 642–647.
Ravi P, Purkait S, Agrawal U, Patra S, Patnaik M, Singh S, dan Mishra P, 2019. Regional Variation of Human Pancreatic Islets Dimension and Its Impact on Beta Cells in Indian Population. Islets; 11(6): 141-151.
Ridwan A, Astrian RT, dan Barlian A, 2012. Measurement of Antidiabetic Effect of Poliphenols( Polyphenon 60 ) Based on Blood Glucose Level and Pancreas. Jurnal Matematika dan Sains; 17(2): 78–82.
Sarita AR dan Kuswanti N, 2022. Pengaruh Pemberian Bubuk Cacing Tanah (Eudrilus eugeniae) terhadap Kadar Glukosa Darah dan Profil Pulau Langerhans Mencit (Mus musculus) Diabetes. LenteraBio; 11(3): 525-535.
Sernita S, Irnawati I, dan Syamsinar S, 2019. Uji Daya Hambat Ekstrak Etanol, Fraksi n- heksana dan Etanol Daun Ceremai (Pyllanthus acidus L.) Skeels) Terhadap Salmonella thypi. BioWallacea: Jurnal Penelitian Biologi (Journal of Biological Research); 6(1).
Setadi E, Peniati E, dan Susanti RS, 2020. Pengaruh Ekstrak Kulit Lidah Buaya Terhadap Kadar Gula Darah Dan Gambaran Histopatologi Pankreas Tikus Yang Diinduksi Aloksan. Life Science; 9(2): 171–185.
Tandi J, Rizky M, Mariani R, dan Alan F, 2017. Uji Efek Ekstrak Etanol Daun Sukun (Artocarpus altilis (Parkinson Ex F.A.Zorn) Terhadap Penurunan Kadar Glukosa Darah,Kolesterol Total Dan Gambaran Histopatologi Pankreas Tikus Putih Jantan (Rattus norvegicus) Hiperkolesterolemia-Diabetes. Jurnal Sains Dan Kesehatan; 1(8): 384–396.
Tatto D, Dewi NP, dan Tibe F, 2017. Efek Antihiperkolesterol dan Antihiperglikemik Daun Ceremai (Phyllantus acidus (L.) Skeels) pada Tikus Putih Jantan (Rattus norvegicus) Hiperkolesterol Diabetes. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal); 3(2): 157–164.
Usman NA, 2018. Pengaruh Ekstrak Kulit Jeruk Lemon (Citrus limon) terhadap Peningkatan Kadar HDL dalam Darah Tikus Putih Jantan (Rattus norvegicus) yang Diinduksi Minyak Goreng Deep Frying. Malang: Universitas Muhammadiyah Malang.
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