Isolation and Characterization of Amylolytic, Proteolytic Bacteria from Papaya Plantation Soil with Attack of Mealybugs (Paracoccus marginatus) in Kebumen

Abstract

Research has been carried out on the isolation of bacteria in papaya plantation soil with mealybugs (Paracoccus marginatus) in Kebumen. This study aims to determine the potential of amylolytic and proteolytic bacteria in papaya plantation soils with mealybug pests. The samples used were soil near the roots of papaya plants with P. marginatus attack, soil on healthy papaya, and soil on dead papaya. Rhizosphere bacteria were isolated and characterized macroscopically and microscopically and carried out enzymatic tests, namely amylolytic tests and proteolytic tests. The isolation results obtained were 8 of the best isolates with the criteria of pure isolates, not contaminated, thriving and producing the desired product, namely with isolate codes B1, B2, B3, B4, B5, B6, B9 and B11. Positive amylolytic test was generated by isolate codes B1, B3, B4, B6. A positive proteolytic test was generated by isolate codes B2, B5, B6, B9. The results of the study proved that the soil in the rhizosphere of papaya plants produced potential bacterial isolates that were amylolytic and proteolytic.