Biokomputasi Aktivitas Senyawa D-alpha-Tocopherol dari Ekstrak Daun Zodia (Evodia suaveolens) sebagai Antikanker secara In Silico
DOI:
https://doi.org/10.26740/lenterabio.v9n2.p129-136Kata Kunci:
kanker, D-alpha-Tocopherol, Antioksidan, In SilicoAbstrak
Kanker merupakan penyakit peringkat ke enam sebagai penyebab kematian terbesar di dunia pada tahun 2016. Prevalensi kanker di Indonesia dari tahun 2013 hingga 2018 meningkat sebanyak 0,6%. Metode pengobatan yang ada saat ini menimbulkan efek samping yang tidak diinginkan, sehingga banyak masyarakat kembali pada pengobatan herbal. Senyawa D-alpha-Tocopherol yang terkandung dalam tanaman zodia (Evodia suaveolens) yang merupakan tanaman endemik Papua memiliki sifat antioksidan yang baik untuk antikanker. Penelitian ini bertujuan untuk mengetahui efektivitas dan bioaktivitas senyawa D-alpha-Tocopherol sebagai antikanker pada kanker paru-paru melalui protein EGFR (Epidermal Growth Factor Reseptor) dan kanker kolorektal melalui protein ALK (Anaplastic Lymphoma Kinase) secara in silico. Metode penelitian yang digunakan yaitu biokomputasi secara in silico, meliputi koleksi protein dan senyawa, sterilisasi dan isolasi protein, minimasi senyawa, docking, dan visualisasi hasil docking antara protein dengan senyawa. Hasil penelitian menunjukkan bahwa senyawa D-alpha-Tocopherol berikatan dengan protein EGFR dengan binding affinity -4,6 KJ/mol; RMSD 1,760 Ã…. Ikatan senyawa D-alpha-Tocopherol dan protein ALK dengan nilai binding affiniy -7,6 KJ/mol; RMSD 2,167. Tingginya nilai binding affinity dan RMSD menunjukkan bahwa penggunaan senyawa D-alpha-Tocopherol sebagai obat antikanker kurang efektif. Sifat antioksidan D-alpha-Tocopherol memberikan keuntungan bagi sel kanker untuk tetap hidup.Referensi
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Basiri MR., Rezazadeh H, Asvadi-Kermani I, Davoodi A, Eghbal MA, Golchin M. and Sarmad M, 2014. Antimutagenic effects of vitamin e on oncology and non oncology hospital nurses by comet assay. Drug Research Vol. 64 (7): 337342. https://doi.org/10.1055/s-0033-1361163.
Bavi P, Jehan Z, Bu R, Prabhakaran S, Al-Sanea N, Al-Dayel F, Al-Kuraya KS, 2013. ALK Gene Amplification is Associated with Poor Prognosis in Colorectal Carcinoma. British Journal of Cancer Vol. 109 (10): 27352743. https://doi.org/10.1038/bjc.2013.641.
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Ghorbani A, Sadeghnia HR. and Asadpour E, 2015. Mechanism of Protective Effect of Lettuce Against Glucose/Serum Deprivation-Induced Neurotoxicity. Nutritional Neuroscience Vol. 18 (3): 103109. https://doi.org/10.1179/1476830513Y.0000000107.
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Held P, 2015. An Introduction to Reactive Oxygen Species Measurement of ROS in Yeast Cells. BioTek Instruments 121. https://doi.org/10.1017/CBO9781107415324.004.
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Isrianto PL, 2016. Bisnis Usaha Perbanyakan Tanaman Zodia ( Evodia suaveolens ) Sebagai Tanaman Pengusir Nyamuk di Kota Surabaya. Inovasi Vol. XVIII (2), 102109.
Khambri D, 2015. Peran terapi hormonal pada kanker payudara. Majalah Kedokteran Andalas Vol. 38: 6473.
Kondoh H, Lleonart ME, Bernard D and Gil J, 2007. Protection from Oxidative Stress by Enhanced Glycolysis; a Possible Mechanism of Cellular Immortalization. Histology and Histopathology Vol. 22(13): 8590. https://doi.org/10.14670/HH-22.85
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Madan R, Benson R, Sharma DN, Julka PK and Rath GK, 2015. Radiation Induced Heart Disease: Pathogenesis, Management and Review Literature. Journal of the Egyptian National Cancer Institute Vol. 27 (4): 187193. https://doi.org/10.1016/j.jnci.2015.07.005.
Muhamat, Wahyuni, T., Rusmiati and Jumar, 2016. Daya Proteksi Minyak Atsiri Zodia (Euvodia suaveolens) dalam Bentuk Spray terhadap Tempat Hinggap Nyamuk Aedes aegypti L. dan Culex quinquefasciatus. Prosiding Seminar Nasional Lahan Basah (1): 278282. Banjarbaru.
Pelicano H, Carney D and Huang P, 2004. ROS Stress in Cancer Cells and Therapeutic Implications. Drug Resistance Updates Vol. 7 (2): 97110. https://doi.org/10.1016/j.drup.2004.01.004.
Purnomo H, 2011. Kimia Komputasi¯: Molecular Docking Plants Penambatan Molekul Plants [Protein-Ligand-Ant-System](œIlmu Semut) (I; Dimaswids, Ed.). Yogyakarta: Pustaka Pelajar.
Radji M, Aldrat, H. Harahap Y and Irawan C, 2010. Pengobatan Obat Herbal Pada Pasien Kanker Serviks. Jurnal Ilmu Kefarmasian Indonesia Vol. 8 (1): 3339.
Rahmatini R, 2015. Evaluasi Khasiat Dan Keamanan Obat (Uji Klinik). Majalah Kedokteran Andalas Vol. 34 (1), 31. https://doi.org/10.22338/mka.v34.i1.p31-38.2010.
Reveal JL and Chase MW, 2009. An Update of the Angiosperm Phylogeny Group Classification for the Orders and Families of Flowering Plants: APG III. Botanical Journal of the Linnean Society 161: 105121. https://doi.org/10.11646/phytotaxa.19.1.4
Saifuddin A, Siswandono and Prajogo EWB, 2014. Studi In Silico Gendarusin A, B, C, D, dan E untuk Prediksi Absorbsi dan Aktivitas terhadap Hialuronidase (Ec 3.2.1.35). Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia Vol. 2 (1): 4247.
Sanora GD, Mastura EY, Handoyo MOM and Purnama ER, 2019. Identification of Anticancer Active Compound from GC-MS Test Results of Zodia Leaves ( Evodia suaveolens ) Ethanol Extract. Jurnal Biota. 5 (2): 8995.
Sattler UGA and Mueller-Klieser W, 2009. The anti-oxidant capacity of tumour glycolysis. International Journal of Radiation Biology Vol. 85 (11): 963971. https://doi.org/10.3109/09553000903258889.
Savard J, Ivers H., Savard MH and Morin CM, 2015. Cancer Treatments and Their Side Effects are Associated with Aggravation of Insomnia: Results of a Longitudinal Study. Cancer Vol. 121 (10): 17031711. https://doi.org/10.1002/cncr.29244
Setiawan SD, 2015. The Effect of Chemotherapy in Cancer Patient to Anxiety. Jurnal Majority 4 (4): 9499.
Simaremare ES, Sinaga DI and Agustini V, 2017. Sabun Zodia Sebagai Repellent Terhadap Nyamuk Aedes aegypti. Pharmaceutical Journal Of Indonesia Vol. 3 (1): 1116.
Smoliga JM., Baur JA and Hausenblas HA, 2011. Resveratrol and Health - A Comprehensive Review of Human Clinical Trials. Molecular Nutrition and Food Research Vol. 55 (8), 11291141. https://doi.org/10.1002/mnfr.201100143.
Suhud F, 2015. Uji Aktivitas In-silico Senyawa Baru 1-Benzil-3-benzoilurea Induk dan Tersubstitusi sebagai Agen Antiproliferatif. Jurnal Farmasi Indonesia Vol. 7 (4): 242251.
Surjadi N and Amtha R, 2013. Radiotherapy Reduced Salivary Flow Rate and Might Induced C. albicans Infection. Journal of Dentistry Indonesia Vol. 19 (1): 16. https://doi.org/10.14693/jdi.v19i1.124.
Suvannang N, Nantasenamat C, Isarankura-Na-Ayudhya C and Prachayasittikul V, 2011. Molecular docking of aromatase inhibitors. Molecules Vol. 16 (5), 35973617. https://doi.org/10.3390/molecules16053597.
Werdhasari A, 2014. Peran Antioksidan Bagi Kesehatan. Indonesian Journal of Biotechnology Medicine Vol. 3(2): 5968. https://doi.org/10.22435/jbmi.v3i2.4203.59-68.
Wijaya CA dan Muchtaridi M, 2017. Pengobatan Kanker Melalui Metode Gen Terapi. Farmaka Vol 15: 5368. https://doi.org/https://doi.org/10.24198/jf.v15i1.12285.g5670.
Yogiyanto dan Sulistianingsih, 2017. Obat Herbal Untuk Penanganan Kemoterapi Dan Efek Samping. Farmaka Vol. 15 (4): 4148.
Zafrial RM dan Amalia R, 2018. Artikel Tinjauan: Anti Kanker dari Tanaman Herbal. Jurnal Ilmiah Farmasi Indonesia Vol. 16 (1): 1523.
Anonim, 2015b. Hormone Therapy to Treat Cancer. Retrieved November 30, 2019, from National Institute of Health website: https://www.cancer.gov/about-cancer/treatment/types/hormone-therapy
Anonim, 2015c. Surgery To Treat Cancer. Retrieved November 30, 2019, from National Institute of Health website: https://www.cancer.gov/about-cancer/treatment/types/surgery
Anonim, 2015d. Types of Cancer Treatment. Retrieved November 27, 2019, from National Institute of Health website: https://www.cancer.gov/about-cancer/treatment/types
Anonim, 2018a. Hasil Utama Riskesdas 2018. In Badan Penelitian dan Pengembangan Kesehatan. https://doi.org/1 Desember 2013
Anonim, 2018b. Latest global cancer data¯: Cancer.
Anonim, 2018c. The Top 10 Causes of Death. Retrieved June 14, 2019, from World Health Organization website: https://www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death
Anonim, 2019. Immunotherapy To Treat Cancer. Retrieved November 30, 2019, from National Institute of Health website: https://www.cancer.gov/about-cancer/treatment/types/immunotherapy
Basiri MR., Rezazadeh H, Asvadi-Kermani I, Davoodi A, Eghbal MA, Golchin M. and Sarmad M, 2014. Antimutagenic effects of vitamin e on oncology and non oncology hospital nurses by comet assay. Drug Research Vol. 64 (7): 337342. https://doi.org/10.1055/s-0033-1361163.
Bavi P, Jehan Z, Bu R, Prabhakaran S, Al-Sanea N, Al-Dayel F, Al-Kuraya KS, 2013. ALK Gene Amplification is Associated with Poor Prognosis in Colorectal Carcinoma. British Journal of Cancer Vol. 109 (10): 27352743. https://doi.org/10.1038/bjc.2013.641.
Du J, Wang XF, Zhou QM, Zhang TL, Lu Y., Zhang H. and Su SB, 2013. Evodiamine induces apoptosis and inhibits metastasis in MDA-MB-231 human breast cancer cells in vitro and in vivo. Oncology Reports Vol. 30 (2): 685694. https://doi.org/10.3892/or.2013.2498.
Engin KN, 2009. Alpha-Tocopherol: Looking Beyond an Antioxidant. Molecular Vision Vol. 15(April), 855860.
Fauziyah A and Dwijananti P, 2013. Pengaruh Radiasi Sinar X Terhadap Motilitas Sperma Pada Tikus Mencit (Mus muculus). Jurnal Pendidikan Fisika Indonesia Vol. 9 (2): 9398.
Ghorbani A, Sadeghnia HR. and Asadpour E, 2015. Mechanism of Protective Effect of Lettuce Against Glucose/Serum Deprivation-Induced Neurotoxicity. Nutritional Neuroscience Vol. 18 (3): 103109. https://doi.org/10.1179/1476830513Y.0000000107.
Hairunnisa H, 2019. Sulitnya Menemukan Obat Baru di Indonesia. Majalah Farmasetika Vol. 4 (1): 16. https://doi.org/10.24198/farmasetika.v4i1.22517.
Hanson ED and Hurley BF, 2011. Intervening on The Side Effects of Hormone-Dependent Cancer Treatment: The Role of Strength Training. Journal of Aging Research, 2011, 18. https://doi.org/10.4061/2011/903291.
Held P, 2015. An Introduction to Reactive Oxygen Species Measurement of ROS in Yeast Cells. BioTek Instruments 121. https://doi.org/10.1017/CBO9781107415324.004.
Horneber M, Bueschel G, Dennert G, Less D, Ritter E and Zwahlen M, 2012. How Many Cancer Patients Use Complementary and Alternative Medicine: A Systematic Review and Metaanalysis. Integrative Cancer Therapies Vol. 11 (3): 187203. https://doi.org/10.1177/1534735411423920.
Isrianto PL, 2016. Bisnis Usaha Perbanyakan Tanaman Zodia ( Evodia suaveolens ) Sebagai Tanaman Pengusir Nyamuk di Kota Surabaya. Inovasi Vol. XVIII (2), 102109.
Khambri D, 2015. Peran terapi hormonal pada kanker payudara. Majalah Kedokteran Andalas Vol. 38: 6473.
Kondoh H, Lleonart ME, Bernard D and Gil J, 2007. Protection from Oxidative Stress by Enhanced Glycolysis; a Possible Mechanism of Cellular Immortalization. Histology and Histopathology Vol. 22(13): 8590. https://doi.org/10.14670/HH-22.85
Liou GY and Storz P, 2010. Reactive Oxygen Species in Cancer. Free Radical Research Vol. 44 (5): 479496. https://doi.org/10.3109/10715761003667554.
Madan R, Benson R, Sharma DN, Julka PK and Rath GK, 2015. Radiation Induced Heart Disease: Pathogenesis, Management and Review Literature. Journal of the Egyptian National Cancer Institute Vol. 27 (4): 187193. https://doi.org/10.1016/j.jnci.2015.07.005.
Muhamat, Wahyuni, T., Rusmiati and Jumar, 2016. Daya Proteksi Minyak Atsiri Zodia (Euvodia suaveolens) dalam Bentuk Spray terhadap Tempat Hinggap Nyamuk Aedes aegypti L. dan Culex quinquefasciatus. Prosiding Seminar Nasional Lahan Basah (1): 278282. Banjarbaru.
Pelicano H, Carney D and Huang P, 2004. ROS Stress in Cancer Cells and Therapeutic Implications. Drug Resistance Updates Vol. 7 (2): 97110. https://doi.org/10.1016/j.drup.2004.01.004.
Purnomo H, 2011. Kimia Komputasi¯: Molecular Docking Plants Penambatan Molekul Plants [Protein-Ligand-Ant-System](œIlmu Semut) (I; Dimaswids, Ed.). Yogyakarta: Pustaka Pelajar.
Radji M, Aldrat, H. Harahap Y and Irawan C, 2010. Pengobatan Obat Herbal Pada Pasien Kanker Serviks. Jurnal Ilmu Kefarmasian Indonesia Vol. 8 (1): 3339.
Rahmatini R, 2015. Evaluasi Khasiat Dan Keamanan Obat (Uji Klinik). Majalah Kedokteran Andalas Vol. 34 (1), 31. https://doi.org/10.22338/mka.v34.i1.p31-38.2010.
Reveal JL and Chase MW, 2009. An Update of the Angiosperm Phylogeny Group Classification for the Orders and Families of Flowering Plants: APG III. Botanical Journal of the Linnean Society 161: 105121. https://doi.org/10.11646/phytotaxa.19.1.4
Saifuddin A, Siswandono and Prajogo EWB, 2014. Studi In Silico Gendarusin A, B, C, D, dan E untuk Prediksi Absorbsi dan Aktivitas terhadap Hialuronidase (Ec 3.2.1.35). Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia Vol. 2 (1): 4247.
Sanora GD, Mastura EY, Handoyo MOM and Purnama ER, 2019. Identification of Anticancer Active Compound from GC-MS Test Results of Zodia Leaves ( Evodia suaveolens ) Ethanol Extract. Jurnal Biota. 5 (2): 8995.
Sattler UGA and Mueller-Klieser W, 2009. The anti-oxidant capacity of tumour glycolysis. International Journal of Radiation Biology Vol. 85 (11): 963971. https://doi.org/10.3109/09553000903258889.
Savard J, Ivers H., Savard MH and Morin CM, 2015. Cancer Treatments and Their Side Effects are Associated with Aggravation of Insomnia: Results of a Longitudinal Study. Cancer Vol. 121 (10): 17031711. https://doi.org/10.1002/cncr.29244
Setiawan SD, 2015. The Effect of Chemotherapy in Cancer Patient to Anxiety. Jurnal Majority 4 (4): 9499.
Simaremare ES, Sinaga DI and Agustini V, 2017. Sabun Zodia Sebagai Repellent Terhadap Nyamuk Aedes aegypti. Pharmaceutical Journal Of Indonesia Vol. 3 (1): 1116.
Smoliga JM., Baur JA and Hausenblas HA, 2011. Resveratrol and Health - A Comprehensive Review of Human Clinical Trials. Molecular Nutrition and Food Research Vol. 55 (8), 11291141. https://doi.org/10.1002/mnfr.201100143.
Suhud F, 2015. Uji Aktivitas In-silico Senyawa Baru 1-Benzil-3-benzoilurea Induk dan Tersubstitusi sebagai Agen Antiproliferatif. Jurnal Farmasi Indonesia Vol. 7 (4): 242251.
Surjadi N and Amtha R, 2013. Radiotherapy Reduced Salivary Flow Rate and Might Induced C. albicans Infection. Journal of Dentistry Indonesia Vol. 19 (1): 16. https://doi.org/10.14693/jdi.v19i1.124.
Suvannang N, Nantasenamat C, Isarankura-Na-Ayudhya C and Prachayasittikul V, 2011. Molecular docking of aromatase inhibitors. Molecules Vol. 16 (5), 35973617. https://doi.org/10.3390/molecules16053597.
Werdhasari A, 2014. Peran Antioksidan Bagi Kesehatan. Indonesian Journal of Biotechnology Medicine Vol. 3(2): 5968. https://doi.org/10.22435/jbmi.v3i2.4203.59-68.
Wijaya CA dan Muchtaridi M, 2017. Pengobatan Kanker Melalui Metode Gen Terapi. Farmaka Vol 15: 5368. https://doi.org/https://doi.org/10.24198/jf.v15i1.12285.g5670.
Yogiyanto dan Sulistianingsih, 2017. Obat Herbal Untuk Penanganan Kemoterapi Dan Efek Samping. Farmaka Vol. 15 (4): 4148.
Zafrial RM dan Amalia R, 2018. Artikel Tinjauan: Anti Kanker dari Tanaman Herbal. Jurnal Ilmiah Farmasi Indonesia Vol. 16 (1): 1523.
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Mastura, E. Y., Asri, M. T., & Purnama, E. R. (2021). Biokomputasi Aktivitas Senyawa D-alpha-Tocopherol dari Ekstrak Daun Zodia (Evodia suaveolens) sebagai Antikanker secara In Silico. LenteraBio : Berkala Ilmiah Biologi, 9(2), 129–136. https://doi.org/10.26740/lenterabio.v9n2.p129-136
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