In-vitro Callus Growth of Amorphophallus muelleri with Corn Organic Compound Supplementation in Murashige and Skoog (MS) Media
DOI:
https://doi.org/10.26740/lenterabio.v15n2.p122-130Keywords:
fresh weight, callus texture, callus colour, plant tissue culture, micropropagation, cropsAbstract
Porang (Amorphophallus muelleri Blume) has prospects as an export commodity as alternative to rice. However, both generative and vegetative propagation remains resulted in limited productivity, thus necessitating alternative propagation through plant tissue culture. This study examined the effect of corn organic compound on the growth of A. muelleri growth in vitro. The research employed a completely randomized design with 0%, 9%, 18%, and 27% concentration of corn filtrate. The texture and colour callus data were analyzed descriptively. Furthermore, the fresh weight of callus was examined using nonparametric Kruskal-Wallis test. The results revealed the callus showed active growth. The callus texture was compact in 9% treatment and friable in 0%, 18%, and 27% treatments. The colour of callus was creamy white in all of the corn filtrate treatments. Callus from the 18% corn filtrate treatment showed the best result, with fresh weight of 5.044 ± 4.627 g but the treatment was not significant statistically compared to other groups. The most optimal callus growth was found in 18% corn filtrate treatment.
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