Phytochemical Profiling of Ethanol Extract from Cocoa Leaves (Theobroma Cacao L.) using Gas Chromatography-Mass Spectrometry

Authors

  • Nadyatul Ilma Indah Savira University of Jember
  • Findiyas Azizah Nurfadhilah Biology Education, University of Jember
  • Sulifah Aprilya Hariani
  • Alfan Irsyad Maulana
  • Amalia Wulandari Biology Education, University of Jember

DOI:

https://doi.org/10.26740/lenterabio.v13n3.p%20377-381

Keywords:

alkaloids, extraction, glycerol, pharmacologist, phenolic

Abstract

Cocoa (Theobroma cacao L.) is one of the highest plantation commodity crops in Indonesia. Previous research showed that cocoa leaves were used as a larvicide, antioxidant, antibacterial, and anticancer. This study aims to determine the phytochemical compounds of ethanol extract of cocoa leaves qualitatively and quantitatively. Extraction of cocoa leaves using maceration method and phytochemical screening using Gas Chromatography-Mass Spectrometry (GC-MS) method. Based on the qualitative phytochemical screening of this study, alkaloid, flavonoid, and saponin tests showed positive results. GC-MS test analysis results showed ethanol extract from cocoa leaves detected 20 compounds. The extract with ethanol solvent produced the most compounds namely glycerol 1,2,3-Propanetriol, 1-acetate (CAS) 1-Acetoxy-2,3-Dihydroxypropane; 1,2,3-Propanetriol, monoacetate; and 1,2,3-Propanetriol, triacetate, phenolic 1,4-Benzenediol (CAS) Hydroquinone, and alkaloid 1H-Indole (CAS) Indole. Therefore, cocoa leaves have many bioactive compounds that have potent pharmacological effects.

References

Abulude FO, Ogunkoya MO, Adenibuyan GB, Arifalo KM, Akinusotu A, Samuel A, and Bello LJ, 2022. Phytochemical assessment of the extracts of stem (Bark) and leaves of theobroma cocoa materials: Experimental procedure and its comparison to literature. ASEAN Journal for Science and Engineering in Materials 1(2): 85-92.

Badan Pusat Statistik Provinsi Jawa Timur, 2023. Produksi Perkebunan Kakao dan Tebu Provinsi Jawa Timur, 2021 dan 2022. https://jatim.bps.go.id/. Diunduh tanggal 29 September 2023.

Baharum Z, Akim AM, Taufiq-Yap YH, Hamid RA, & Kasran R, 2014. In vitro antioxidant and antiproliferative activities of methanolic plant part extracts of Theobroma cacao. Molecules, 19(11): 18317-18331.

Diaz AFO, Gonzalez YP, Barraza JC, Hill C, Fletcher WQ, Verbel J and Castillo FD, 2022. Larvicidal Activity of Plant Extracts from Colombian North Coast Against Aedes aegypti L. Mosquito Larvae. Arabian Journal of Chemistry 15 (12): 104365.

Indrianingsih, A. W., Wulanjati, M. P., Windarsih, A., Bhattacharjya, D. K., Suzuki, T., & Katayama, T., 2021. In vitro studies of antioxidant, antidiabetic, and antibacterial activities of Theobroma cacao, Anonna muricata and Clitoria ternatea. Biocatalysis and Agricultural Biotechnology 33, 101995.

Komlaga G, Forkuo AD, Suleman N, Nkrumah D, Nketia R, & Bekoe SO, 2021. Antimalarial Property and Acute Toxicity of the Leaves of Theobroma cacao L. Evidence-based Complementary and Alternative Medicine, 2021, 2852442.

Omar AF, El-Naggar MM, Talat D, Saleh H, and Ibrahim MS, 2021. Anti-Salmonella Products from Immobilized Marine Bacillus amyloliquefaciens AMM. International Journal of Pharmaceutical Research (09752366) 13 (2).

Osman H, Nasarudin R, & Lee SL, 2004. Extracts of cocoa (Theobroma cacao L.) leaves and their antioxidation potential. Food chemistry, 86 (1): 41-46.

Parbuntari, H., Prestica, Y., Gunawan, R., Nurman, M. N., & Adella, F. 2018. Preliminary phytochemical screening (qualitative analysis) of cacao leaves (Theobroma cacao L.). EKSAKTA: Berkala Ilmiah Bidang MIPA 19(2), 40-45.

Singh N, Datta S, Dey A, Chowdhury AR, & Abraham, J. 2015. Antimicrobial activity and cytotoxicity of Theobroma cacao extracts. Der Parmacia Lettre 7(7): 287-294.

Downloads

Published

2024-07-04

How to Cite

Savira, N. I. I., Nurfadhilah , F. A. ., Hariani, S. A., Maulana, A. I., & Wulandari, A. (2024). Phytochemical Profiling of Ethanol Extract from Cocoa Leaves (Theobroma Cacao L.) using Gas Chromatography-Mass Spectrometry. LenteraBio : Berkala Ilmiah Biologi , 13(3), 377–381. https://doi.org/10.26740/lenterabio.v13n3.p 377-381
Abstract views: 226 , PDF Downloads: 227