Aktivitas Urease dan Pembentukan Kalsium Karbonat oleh Bakteri Ureolitik

Authors

  • Tetty Marta Linda Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau
  • Mufidah Dwi Suci Ningsih Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau
  • Bernadeta Leni Fibriarti Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau
  • Saras Andini Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau
  • Dedi Futra Program Studi Pendidikan Kimia, Fakultas Kejuruan dan Ilmu Pengetahuan, Universitas Riau

DOI:

https://doi.org/10.26740/lenterabio.v11n1.p139-143

Keywords:

Bacillus, urease, kalsium karbonat

Abstract

Bakteri ureolitik adalah bakteri yang memiliki aktivitas enzim urease yang menghidrolisis urea untuk menghasilkan karbondioksida dan amonia. Bakteri ini dapat diaplikasikan untuk agrikultur, kesehatan dan konstruksi. Penelitian ini bertujuan untuk melihat aktivitas urease Bacillus SP 34 dan Bacillus SP 83 dan kemampuannya menghasilkan kalsium karbonat. Pengujian aktivitas urease secara kuantitatif dilakukan dalam Nutrient Broth selama 18 jam. Produksi kalsit menggunakan medium NB-U/Ca inkubasi selama 7 hari.  Hasil penelitian diketahui Bacillus SP 34  aktivitas urease yaitu 0,058 U/ml dan kalsium karbonat terbentuk  169.0 mg dan Bacillus SP 83 dengan aktivitas urease 0.065 U/ml dan kalsium karbonat terbentuk 105.0 g.  Ke dua isolat ini dapat dikembangkan untuk bidang pertanian dan  konstruksi sebagai bahan campuran beton.

Author Biography

Tetty Marta Linda, Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau

Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau.

 

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Published

2021-11-30

How to Cite

Linda, T. M., Ningsih, M. D. S., Fibriarti, B. L., Andini, S., & Futra, D. (2021). Aktivitas Urease dan Pembentukan Kalsium Karbonat oleh Bakteri Ureolitik. LenteraBio : Berkala Ilmiah Biologi, 11(1), 139–143. https://doi.org/10.26740/lenterabio.v11n1.p139-143

Issue

Vol. 11 No. 1 (2022): Volume 11 Nomor 1

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Artikel

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