Papaya Seeds (Carica papaya L.) and Lemongrass (Cymbopogon citratus) Decoction Ameliorated Mice (Mus musculus) with Type II Diabetes Mellitus
DOI:
https://doi.org/10.26740/lenterabio.v15n3.p197-204Keywords:
non-communicable disease, fasting blood glucose, high fat diet, pancreasAbstract
The antioxidant activity of papaya seed and lemongrass can possibly lower blood glucose levels and improve the Langerhans islet in mice with type 2 diabetes mellitus (T2DM) induced by high-fat diet (HFD) and alloxan. This study aimed to evaluate the effect of papaya seed and lemongrass stem decoctions on fasting blood glucose (FBG) levels and diameter of Langerhans islets, as well as to identify the optimal decoction for both parameters. This experimental study used 24 male mice divided into 6 treatment groups: negative control (KN), T2DM-induced positive control (KP), T2DM with metformin (KM), 60% papaya seed decoction (KA), 60% lemongrass decoction (KB), and 60% combined papaya seed and lemongrass decoction (KC). The FBG data were analyzed using Brown-Forsythe test and Games-Howell test. Meanwhile, diameter data of Langerhans islet were analyzed using ANOVA and Duncan test. The results showed that the KB group was the most effective decoction in reducing FBG from 166.5 ± 21.05 mg/dL to 111.25 ± 14.66 mg/dL and improving the diameter of Langerhans islet to 133.29 ± 14.66 μm.
References
Amin S, Saraswati E, Ilhami DAD, Muliadi P, Anggraeni YD, and Pratama A, 2025. Mekanisme Kerja Obat Anti Diabetes Golongan Biguanid Dan Sulfonilurea Tinjauan Kimia Medisinal. Jurnal Ners, 9(2):2981-2986.
Bailey CJ, 2017. Metformin: Historical Overview. Diabetologia, 60:1566-1576.
Choo YN, Ravi RN, and Subramaniyan V, 2026. Insulin Resistance Induced by Obesity: Mechanisms, Metabolic Implications, and Therapeutic Approaches. Molecular Biology Reports, 53:357.
Elkanawati RY, Sumiwi SA, and Levitta J, 2024. Impact of Lipids on Insulin Resistance: Insights From Human and Animal Studies. Drug Design, Development, and Therapy, 18:3337-3360.
Feng Z, Tan Z, and Lu D, 2025. Mitochondrial Bioenergetics Dysfuncion in T2DM: Linking Oxidative Stress to Insulin Resistance. Frontiers in Endocrinology, 16:1674477.
Gazwi HSS, 2019. Preventive Effect of Lemongrass (Cymbopogon citratus) Agains Oxidation in Soybean Oil. Proceedings of the National Academy of Sciences, India Section B: Biological Science, 90:151-159.
Halim M and Halim A, 2019. The Effects of Inflammation, Aging, Oxidative Stress on The Pathogenesis of Diabetes Mellitus (Type 2 Diabetes). Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 13(2):1165-1172.
Hanna DN and Qomariyah N, 2025. Effect of High-Fat Diet Variations on Cholesterol Levels and Hepatic Histopathology in Mouse Models of Type 2 Diabetes Mellitus. LenteraBio, 14(3):303-310.
International Diabetes Federation, 2021. IDF Diabetes Atlas (10th edition). International Diabetes Federation.
International Diabetes Federation, 2025. IDF Diabetes Atlas (11th edition). International Diabetes Federation.
Khaleyla F, Ducha N, dan Bahsri A, 2021. Metode Parafin untuk Sediaan Irisan Jaringan Hewan. Jurusan Biologi Unesa.
Khin PP, Lee JH, and Jun HS, 2023. Pancreatic Beta-Cell Dysfunction in Type 2 Diabetes. European Journal of Inflammation, 21:1-13.
Mahmuda R, Himaniarwati H, and Imran SAA, 2024. Standarisasi dan Uji Aktivitas Antikolesterol Ekstrak Etanol Daun Labu Kuning (Curcubita moschata Duch.) pada Mencit (Mus musculus). Journal Mandala Pharmacon Indonesia, 10(1):111-121.
Nubatois DW, Ndaong NA, and Selan YN, 2015. Pengaruh Pemberian Ekstrak Etanol Daun Sambiloto (Andrographis paniculata Nees) Terhadap Histopatologi Pankreas Mencit (Mus musculus) Diabetes Melitus (DM) Tipe I. Jurnal Kajian Veteriner, 3(1):31-40.
Perkumpulan Endokrinologi Indonesia (PERKENI), 2019. Pedoman Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 di Indonesia. Jakarta: PB PERKENI.
Petersen MC and Shulman GI, 2018. Mechanism of Insulin Action and Insulin Resistance. Physiological Reviews, 98:2133-2223.
Pokhrel S and Karki S, 2021. Phytochemical Screening, Antioxidant and Antidiabetic Activities of Extract of Leaves and Seeds of Carica papaya. Nepal Journal of Science and Technology, 20(1):126-135.
Rahayu S, Julaiha S, Ardini D, and Indriyani DM, 2024. Gambaran Efek Samping Obat Berdasarkan Usia, Cara Minum, dan Dosis Obat Metformin Pada Penderita Diabetes Melitus Tipe 2. Journal Pharmaceutical Sciences and Technology, 1(1):7-16.
Rahman S, Kosman R, and Sudrianto S, 2015. Uji Aktivitas Antioksidan Kombinasi Infusa Biji alpukat (Persea Americana) dan Biji Pepaya (Carica papaya L) Terhadap Tikus Putih (Rattus norvegicus) Diabetes Melitus Dengan Parameter MDA. As-Syifaa, 7(1):34-42.
Ridho FM, 2023. Mechanism of alkaloids and Flavonoids in Bajakah (Uncaria nervosa Elmer) as Antidiabetic Agents. Jurnal Ilmu Media Indonesia (JIMI), 3(1):9-16.
Röhrborn D, Wronkowitz N, and Eckel J, 2015. DPP4 in Diabetes. Frontiers in Immunology, 6:386.
Sari T, Gracienne, Hariesti RA, Destra E, Gunaidi FC, and Firmansyah Y, 2024. Edukasi dan Skrining Pentingnya Pemantauan Obesitas terhadap Terjadinya Resistensi Insulin Pada Lanjut Usia. Jurnal Pengabdian Bidang Kesehatan, 2(2):64-72.
Samuel VT and Shulman GI, 2016. The Pathogenesis of Insulin Resistance Integrating Signaling Pathways and substrate Flux. The Journal of Clinical Investigation, 126(1):12-22.
Shahidi F and Danielski R, 2024. Review on The Role of Polyphenols in Preventing and Treating Type 2 Diabetes: Evidence From In Vitro and In Vivo Studies. Nutrient, 16(18):3159.
Shou J, Chen PJ, and Xiao WH, 2020. Mechanism of Increase Risk of Insulin Resistance in Aging Skeletal Muscle. Diabetology and Metabolic Syndrome Chemistry, 12(1):1-10.
Sieniawska E, 2015. Activities of Tannins-From In Vitro Studies to Clinical Trials. Natural Product Communication, 10(11):1877-1884.
Smeriglio A, Barreca D, Bellocco E, and Trombetta D, 2017. Proanthocyanidins and Hydrolysable Tannins: Occurrence, Dietary Intake , and Pharmacological Effects. Bristish Journal of Pharmacology, 174(11):1244-1262.
Susanti S and Bistara DN, 2023. Effectivity Between Lemongrass Stew and Cinnamon Infusion in Reducing Blood Glucose Level in Diabetes Mellitus Patients. Lux Mensana: Journal of Scientific Health; 2(3):170-179.
Tang W, Liu H, Deng S, and Gao C, 2026. Influence and Treatment of Insulin Receptor Substrate/PI3K/Akt-Mediated Insulin Resistance in Diabetes Meliitus (Review). Molecular Medicine Reports, 33(2):1-13.
Utama Z, Ningrum A, Mayangsari Y, and Rajharjo S, 2024. A Comparison of Palm Oil, Beef Tallow, and Corn Oil Effect on Serum Glucose and Lipid Profile in Rats Fed a High-Fat Diet. AgriTECH, 44(2):180.188.
Widiasari KR, Wijaya IMK, and Suputra PA, 2021. Diabetes Melitus Tipe 2: Faktor Risiko, Diagnosis, dan Tatalaksana. Ganesha Medicina, 1(2):114-120.
Widaryanti B and Tripramatasari FL, 2021. Efek Rebusan Sereh (Cymbopogon citratus) Terhadap Kadar Glukosa dan Profil Lipid Tikus Wistar Diabetes. BioWallacea: Jurnal Peneltian Biologi (Journal of Biological Research), 8(1)-1-9
Wulansari DD, Basori A, and Suhartati, 2017. Pengaruh Pemberian ekstrak Biji Pepaya (Carica papaya Linn.) terhadap Ekspresi Glucose Transporter i4 (GLUT 4) pada Jaringan Otot Rangka Tikus Model Diabetes Melitus Tipe II yang Diinduksi Diet Tinggi. Traditional Medicine Journal, 22(2):131-138.
Zhang QW, Lin LG, and Ye WC, 2018. Echniques for Extraction and Isolation of Natural Product:A Comprehensive Review. Chinese Medicine, 13:20.
Zulkarnaini A, Mahatma G, Puspita D, Vani AT, and Abdullah D, 2022. Aktivitas Fisik, Pola makan, dan Konsumsi Makanan Glikemik Tinggi Meningkatkan Risiko Kejadian Diabetes Mellitus Tipe 2. Jurnal Kesehatan Metro Se Wawai, 15(2):154-162.
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