Effect of Moringa Leaf and Shallot Filtrate in MS Media on the Growth of Sambung Nyawa (Gynura procumbens) Plantlets
DOI:
https://doi.org/10.26740/lenterabio.v15n2.p161-167Keywords:
moringa, natural growth regulator, planlet, sambung nyawa, shallots , tissue cultureAbstract
Sambung nyawa (Gynura procumbens)is a local plant that sees a lot of uses, however its cultivation is still limited. Thus, tissue culture can be examined as potential propagation methods. This study determined the effect of moringa leaves and shallots filtrate to Murashige and Skoog (MS) media on sambung nyawa plantlets’s growth. This study was an experimental with 2 combined factors; moringa leaves and shallots filtrate at various concentration and BAP and IAA control treatment. The growth parameters observed were plantlet height, leaves’s number, root length and roots’s number. The data obtained were then tested for normality, Friedman Test, and Mann-Whitney Test. This study found that there was effect on varying concentration of moringa leaf filtrate, concentration of shallot filtrate, and the interaction of both on the growth of sambung nyawa plantlets with 37.5 g/L moringa leaf filtrate resulted in best plantlet height, 37.5 g/L shallot filtrate had the greatest number of leaves, 50 g/L shallot filtrate resulted in highest root length and combination of 25 g/L moringa leaf and 37.5 g/L shallot filtrated resulted in the highest number of roots.
References
Apriliyani R, and Wahidah BF, 2021. Perbanyakan anggrek Dendrobium sp. secara in vitro: Faktor-faktor keberhasilannya. Filogeni: Jurnal Mahasiswa Biologi 1(2): 33–46.
Aziz MM, Ratnasari E, and Rahayu YS, 2014. Induksi Kalus Umbi Iles-Iles (Amorphophallus muelleri) dengan Kombinasi Konsentrasi 2, 4-D dan BAP Secara In Vitro. LenteraBio 3(2): 109–114.
Bakhtra DD, Jubahar J, and Yusdi E, 2018. Uji Aktivitas Fraksi Dari Ekstrak Daun Sambung Nyawa (Gynura procumbens (Lour) Merr.) Terhadap Bakteri Shigella dysenteriae. Jurnal Farmasi Higea 10(1): 10–18.
Benkeblia N, 2004. Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). LWT-Food Science and Technology 37(2): 263–268.
Culver M, Fanuel T, and Chiteka AZ, 2012. Effect of moringa extract on growth and yield of tomato. Greener Journal of Agricultural Sciences 2(5): 207–211.
Farman S, Mushtaq A, and Azeem MW, 2019. Plant growth regulators (PGRs) and their applications: A review. International Journal of Chemical and Biochemical Sciences 15: 94-103.
Gresiyanti DM, Anissa RK, Setyawati FD, Susanto AD, and Ratnasari E, 2021. Perbandingan Efektivitas Ekstrak Bawang Merah dan Auksin Sintetik Terhadap Pertumbuhan Akar Jagung (Zea mays L.). Prosiding Seminar Nasional Biologi 1: 715–724.
Hasibuan AS, Edrianto V, and Purba N, 2020. Skrining fitokimia ekstrak etanol umbi bawang merah (Allium cepa L.). Jurnal Farmasimed (JFM) 2(2): 45–49.
Howladar SM, 2014. A novel Moringa oleifera leaf extract can mitigate the stress effects of salinity and cadmium in bean (Phaseolus vulgaris L.) plants. Ecotoxicology and Environmental Safety 100: 69–75.
Iqbal J, Irshad J, Bashir S, Khan S, Yousaf M, and Shah AN, 2020. Comparative study of water extracts of Moringa leaves and roots to improve 52 the growth and yield of sunflower. South African Journal of Botany, 129: 221-224.
Kaffi U, 2018. Uji Efektifitas Pertumbuhan Vegetatif Bunga Nusa Indah (Mussaenda Pubescens) Terhadap Pemberian ZPT Organik Jagung Muda Pada Berbagai Sumber Setek. AGROVITAL: Jurnal Ilmu Pertanian 2(2): 62–66.
Manuhara YSW, Noviyanti R, Hardjo PH, and Tanjung M, 2019. Enhancement of flavonoid production of Gynura procumbens (Lour.) Merr adventitious roots in baloon-type bubble bioreactor influenced by phenylalanine and tyrosine. Ecology Environment dan Conservation Journal 25: S61–S66.
Majumder S, Biswas A, and Rahman MM, 2016. In vitro mass propagation of Gynura procumbens (Lour.) Merr.-an important medicinal plant. Asian Journal of Natural & Applied Sciences 5(3): 71-79.
Mutryarny E, Endriani E, and Purnama I, 2022. Efektivitas Zat Pengatur Tumbuh dari Ekstrak Bawang Merah pada Budidaya Bawang Daun (Allium porum L). Jurnal Pertanian 13(1): 33–39.
Nasir M, Khan AS, Basra SMA, and Malik AU, 2016. Foliar application of moringa leaf extract, potassium and zinc influence yield and fruit quality of ‘Kinnow’mandarin. Scientia Horticulturae 210: 227–235.
Nurcahyani E, Zulkifli Z, and Kanedi M, 2021. Pengenalan dan Pelatihan Teknik Kultur Jaringan Tumbuhan Bagi Guru Biologi SMA Se-Kabupaten Tanggamus Provinsi Lampung. Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN 2(1): 39–46.
Rainiyati L, and Kristiana M, 2009. Peranan IAA dan BAP terhadap perkembangan nodul pisang (Musa AAB) raja nangka secara in vitro. Jurnal Agronomi 13(1): 51–57.
Roychoudhry S, and Kepinski S, 2022. Auxin in root development. Cold Spring Harbor Perspectives in Biology 14(4): a039933.
Sagar NA, Pareek S, Benkeblia N, and Xiao J, 2022. Onion (Allium cepa L.) bioactives: Chemistry, pharmacotherapeutic functions, and industrial applications. Food Frontiers 3(3): 380–412.
Saini RK, Sivanesan I, and Keum YS, 2016. Phytochemicals of Moringa oleifera: a review of their nutritional, therapeutic and industrial significance. 3 Biotech 6: 1–14.
Sakinah DG, Putra ETS, and Rogomulyo R, 2018. Produksi dan kadar flavonoid daun sambung nyawa (Gynura procumbens (lour.) merr.) pada tiga fase agroforestri. Vegetalika 7(3): 1-15.
Salisbury FB, and Ross CW, 1992. Plant physiology. Belmont: Wadsworth Publishing.
Svolacchia, N, and Sabatini, S, 2023. Cytokinins. Current Biology 33(1): R10-R13.
Tarigan PL, and Nurbaiti SY, 2017. Pemberian ekstrak bawang merah sebagai zat pengatur tumbuh alami pada pertumbuhan setek lada (Piper nigrum L.). Jurnal Online Mahasiswa (JOM) FAPERTA 1: 1-11.
Tini EW, Sakhidin S, Saparso S, and Haryanto TAD, 2022. Perbandingan Hormon Endogenous pada Beberapa Tanaman. Jurnal Galung Tropika 11(2): 132–142.
Wahyuni A, Satria B, and Zainal A, 2020. Induksi kalus gaharu dengan NAA dan BAP secara in vitro. Agrosains: Jurnal Penelitian Agronomi 22(1): 39 44.
Widiastoety D, 2014. Pengaruh auksin dan sitokinin terhadap pertumbuhan planlet anggrek Mokara (Effect of auxin and cytokinin on the growth of Mokara orchid plantlets). Journal of Tropical Horticulture 24(3): 230–238.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 LenteraBio : Berkala Ilmiah Biologi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Hak Cipta (c) LenteraBio: Berkala Ilmiah Biologi
Karya ini dilisensikan di bawah Lisensi Internasional Creative Commons Attribution-NonCommercial 4.0.
Pemberitahuan Hak Cipta.
Hak cipta dari artikel yang diterima untuk diterbitkan akan diberikan kepada jurnal sebagai penerbit jurnal. Hak cipta yang dimaksud meliputi hak untuk menerbitkan artikel dalam berbagai bentuk (termasuk cetak ulang). Jurnal mempertahankan hak penerbitan atas artikel yang diterbitkan.
Abstract views: 0
,
PDF Downloads: 0
