Effect of Cavendish Banana Filtrates and BAP (6-Benzylaminopurine) in Vacin and Went (VW) Media on the Growth of Cattleya sp. Orchid Planlet In Vitro
DOI:
https://doi.org/10.26740/lenterabio.v15n1.p58-63Keywords:
leaves, tissue culture, plantlet height, leaf color, plant speciesAbstract
The production of Cattleya sp. orchids has declined due to environmental changes and limitations in cultivation techniques, necessitating propagation methods using in vitro tissue culture. This study aims to determine the effect and optimal concentration of Cavendish banana filtrate and BAP on VW medium for in vitro growth of Cattleya orchid planlets. The research used a completely randomized design with five treatments and five replications; negative control (K), P1 (10g/L Cavendish banana filtrate + 2 ppm BAP), P2 (15g/L Cavendish banana filtrate + 1.5 ppm BAP), P3 (20g/L Cavendish banana filtrate + 1 ppm BAP), and P4 (25g/L Cavendish banana filtrate + 0.5 ppm BAP). Growth was observed from parameters including the number of leaves, leaf color, and plantlet height, which were analyzed using one-way ANOVA and Duncan's multiple range test. Results show that P1 (10g/L Cavendish banana filtrate + 2 ppm BAP) and P2 (15g/L Cavendish banana filtrate + 1.5 ppm BAP) had optimal effect on number and color of leaves, while P3 (20g/L Cavendish banana filtrate + 1 ppm BAP) and P4 (25g/L Cavendish banana filtrate + 0.5 ppm BAP) resulted on best height of Cattleya orchid planlets.
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