Antibacterial Activity of Beluntas (Pluchea indica L.) Leaves Extract using Different Extraction Methods
DOI:
https://doi.org/10.26740/jrba.v2n2.p49-54Keywords:
Antibacterial activity, beluntas leaves, soxhletation methodAbstract
Escherichia coli and Bacillus subtilis are bacteria that cause disease in the digestive tract. Pluchea indica L. had the pharmacological activity of antiseptic power against bacteria that cause digestive tract infections because of contained antibacterial compounds. The study on the comparison of extraction methods will enable the public to choose a better extraction method to use as an antibacterial agent. This study aimed to identify the content of antibacterial compounds in Pluche indica L. and to determine the antibacterial ability of Pluche indica L. extract using maceration, percolation, and soxhletation methods. The results showed that Pluchea indica L. contains flavonoids, alkaloids, saponins, and tannins. The highest diameter inhibition of Escherichia coli and Bacillus subtilis was obtained from Pluchea indica L. leaves extract extracted by using the Soxhletation method.
References
Agoes, A., (2010). Tanaman Obat Indonesia. Jakarta: Salemba Medika.
Bettelheim, K. A. (2000). Role of non-0157 VTEC. Journal of Applied Microbiology Symposium Supplement, 88(S1), 38S-50S. https://doi.org/https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2672.2000.tb05331.x.
Dalimartha, S. (1999). Atlas Tumbuhan Obat Indonesia. Jilid I. Jakarta: Trubus Agriwidya.
Endang & Maimunah. (2018). Uji Efektivitas Air Buah Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Candida albicans (Studi di STIkes ICMe Jombang). Karya Tulis Ilmiah. http://repo.stikesicme-jbg.ac.id/1477/2/151310011_Endang%20Maimunah_%20KTI%20PDF.pdf.
Hafsari, A.R., Cahyanto, T., Sujarwo, T (2015). Uji Aktivitas Antibakteri Daun Beluntas (Pluchea indica (L.) LESS. ) terhadap Propionibacterium acnes Penyebab Jerawat Anggita. Journal Istek, IX(1), 142161. https://journal.uinsgd.ac.id/index.php/istek/article/view/174/191
Ismi, R., Ratnawati, D.M. and Yudi, R. (2010). Uji Aktifitas Antibakteri Fraksi Aktif Ekstrak Etanolik Daun Beluntas (Pluchea indica L.) dengan Metode Maserasi dan Soxhletasi terhadap Salmonella typhi atcc 13311 Secara Dilusi. Biomedika, 1(2). http://portalgaruda.org/?ref=browse&mod=viewarticle&article=152949.
Jawetz, E. (1995). Mikrobiologi Kedokteran. Terjemahan Edi Nugroho dan Maulany dari Medical Microbiology (1995). Jakarta : EGC.
Khotimah, K.(2016) Skrining fitokimia dan identifikasi metabolit sekunder senyawa karpain pada ekstrak metanol daun Carica Pubescens Lenne & K. Koch dengan LC/MS (Liquid Chromatograph-tandem Mass Spectrometry). Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.
Koirewoa, Y. A., Fatimawali, & Wiyono, W. I. (2012). Isolasi dan Identifikasi Senyawa Flavonoid Dalam Daun Beluntas (Pluchea indica L.). Pharmacon, 1(1), 4752. https://doi.org/https://doi.org/10.35799/pha.1.2012.445.
Kurniawati, M. (2001). Uji Aktivitas Antibakteri Fraksi Daun Sirsak (Anonna murrcata) terhadap Staphylococcus aureus dan Escherichia coli serta Profil Kromatografi Lapis Tipusnya. Skripsi Fakultas Farmasi Gadjah Mada, Yogyakarta.
Manu, R. R. S. (2013). Aktivitas Antibakteri Ekstrak Etanol Daun Beluntas (Pluchea Indica L.) terhadap Staphylococcus Aureus, Bacillus Subtilis dan Pseudomonas aeruginosa. Calyptra: Jurnal Ilmiah Mahasiswa Universitas Surabaya, 2(1), 110. http://www.journal.ubaya.ac.id/index.php/jimus/article/view/162/139
Mulyadi, A. F., Pulungan, H., & Qayyum, N. (2016). Pembuatan Edible Film Maizena dan Uji Aktifitas Antibakteri (Kajian Konsentrasi Gliserol dan Ekstrak Daun Beluntas (Pluchea Indica L.) Producing of Cornstarch Edible film and Antibacterial Activity Test (The Study of Glycerol Concentration and Beluntas L. Industria: Jurnal Teknologi Dan Manajemen Agroindustri, 5(3), 149158. https://doi.org/https://doi.org/10.21776/ub.industria.2016.005.03.5
Nurhalimah, H., Wijayanti, N., & Widyaningsih, T. D. (2015). Efek Antidiare Ekstrak Daun Beluntas pada Mencit. Jurnal Pangan dan Agroindustri, 3(3), 10831094. https://jpa.ub.ac.id/index.php/jpa/article/view/231.
Pan, X., Chen, F., Wu, T., Tang, H., & Zhao, Z. (2009). The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Food Control, 20(6), 598602. https://doi.org/10.1016/j.foodcont.2008.08.019.
Paz, J.E.W., Contreras, C.R., Munguia, A.R., Aguilar, C.R., Inungaray, M.L.C. (2017). Phenolic content and antibacterial activity of extracts of Hamelia patensobtained by different extraction methods. Braz J Microbiol. 49(3), 656661.
Puspitasari, S. A., & Proyogo, L. S. (2017). Kadar Fenolik Total Ekstrak Etanol Daun Kersen (Muntingia calabura). Jurnal Ilmiah Cendekia Eksakta, 2(1), 18. https://doi.org/Perbandingan Metode Ekstraksi Maserasi Dan Sokletasi Terhadap Kadar Fenolik Total Ekstrak Etanol Daun Kersen (Muntingia calabura).
Prayoga, E. (2013). Perbandingan Efek Ektrak Daun Sirih Hijau (Piper betle L) Dengan Metode Difusi Disk dan Sumuran Terhadap Bakteri Staphylococcus aureu. Tesis. 1-3. Universitas Islam Negeri Syarif Hidayatullah Jakarta.
Syukur,R., Alam, G., Mufidah., & Rahim, R. T. (2011). Aktivitas Antiradikal Bebas Beberapa Ekstrak Tanaman Familia Fabaceae Radical. Kesehatan, 1(1), 6167. http://pasca.unhas.ac.id/jurnal/files/e72a6e54ecc12a1c6d06853e9e5cd778.pdf.
Radji and Maksum., (2011). Buku Ajar Mikrobiologi Panduan Mahasiswa Farmasi & Kedokteran. Jakarta: Kedokteran ECG.
Robinson, T. 1991. Kandungan Organik Tumbuhan Tingkat Tinggi. Diterjemahkan oleh Prof. Dr. Kosasih Padmawinata. Bandung: Penerbit ITB.
Amilah, S & Ajiningrum, P.S. (2015). Uji Efektivitas Daya Hambat Sari Daun Pegagan (Centella asiatica) dan Daun Beluntas (Pluchea indica Less) Terhadap Pertumbuhan Mycobacterium tuberculosis. Stigma Journal of Science, 8(2), 611. https://doi.org/https://doi.org/10.36456/stigma.vol8.no02.a253
Suru, E., Yamlean, P. V. Y., & Lolo, W. A. (2019). Formulasi dan Uji Efektivitas Krim Antibakteri Ekstrak Etanol Daun Beluntas (Pluchea indica Less .) terhadap Bakteri Propionibacterium acnes. PHARMACON, 8(2), 209218. https://doi.org/https://doi.org/10.35799/pha.8.2019.29256.
Thompson, E.B. (1985). Drug Biosreening. America: Graceway Publishing Company, Inc, Pp, 40, 118
Widyawati, P. S., WIjaya, C. H., Hardjosworo, P. S., & Sajuthi, D. (2011). Evaluasi Aktivitas Antioksidatif Ekstrak Daun Beluntas (Plucea indica) Berdasarkan Perbedaan Ruas Daun. Jurnal Teknologi Pangan, 5(1), 117. https://core.ac.uk/download/pdf/234615371.pdf.
Zein, U. (2004). Diare Akut Disebabkan Bakteri. E-USU Repository, January 2004, 115.https://www.researchgate.net/publication/42321299_Diare_Akut_Disebabkan_Bakteri.
Bettelheim, K. A. (2000). Role of non-0157 VTEC. Journal of Applied Microbiology Symposium Supplement, 88(S1), 38S-50S. https://doi.org/https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2672.2000.tb05331.x.
Dalimartha, S. (1999). Atlas Tumbuhan Obat Indonesia. Jilid I. Jakarta: Trubus Agriwidya.
Endang & Maimunah. (2018). Uji Efektivitas Air Buah Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Candida albicans (Studi di STIkes ICMe Jombang). Karya Tulis Ilmiah. http://repo.stikesicme-jbg.ac.id/1477/2/151310011_Endang%20Maimunah_%20KTI%20PDF.pdf.
Hafsari, A.R., Cahyanto, T., Sujarwo, T (2015). Uji Aktivitas Antibakteri Daun Beluntas (Pluchea indica (L.) LESS. ) terhadap Propionibacterium acnes Penyebab Jerawat Anggita. Journal Istek, IX(1), 142161. https://journal.uinsgd.ac.id/index.php/istek/article/view/174/191
Ismi, R., Ratnawati, D.M. and Yudi, R. (2010). Uji Aktifitas Antibakteri Fraksi Aktif Ekstrak Etanolik Daun Beluntas (Pluchea indica L.) dengan Metode Maserasi dan Soxhletasi terhadap Salmonella typhi atcc 13311 Secara Dilusi. Biomedika, 1(2). http://portalgaruda.org/?ref=browse&mod=viewarticle&article=152949.
Jawetz, E. (1995). Mikrobiologi Kedokteran. Terjemahan Edi Nugroho dan Maulany dari Medical Microbiology (1995). Jakarta : EGC.
Khotimah, K.(2016) Skrining fitokimia dan identifikasi metabolit sekunder senyawa karpain pada ekstrak metanol daun Carica Pubescens Lenne & K. Koch dengan LC/MS (Liquid Chromatograph-tandem Mass Spectrometry). Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim.
Koirewoa, Y. A., Fatimawali, & Wiyono, W. I. (2012). Isolasi dan Identifikasi Senyawa Flavonoid Dalam Daun Beluntas (Pluchea indica L.). Pharmacon, 1(1), 4752. https://doi.org/https://doi.org/10.35799/pha.1.2012.445.
Kurniawati, M. (2001). Uji Aktivitas Antibakteri Fraksi Daun Sirsak (Anonna murrcata) terhadap Staphylococcus aureus dan Escherichia coli serta Profil Kromatografi Lapis Tipusnya. Skripsi Fakultas Farmasi Gadjah Mada, Yogyakarta.
Manu, R. R. S. (2013). Aktivitas Antibakteri Ekstrak Etanol Daun Beluntas (Pluchea Indica L.) terhadap Staphylococcus Aureus, Bacillus Subtilis dan Pseudomonas aeruginosa. Calyptra: Jurnal Ilmiah Mahasiswa Universitas Surabaya, 2(1), 110. http://www.journal.ubaya.ac.id/index.php/jimus/article/view/162/139
Mulyadi, A. F., Pulungan, H., & Qayyum, N. (2016). Pembuatan Edible Film Maizena dan Uji Aktifitas Antibakteri (Kajian Konsentrasi Gliserol dan Ekstrak Daun Beluntas (Pluchea Indica L.) Producing of Cornstarch Edible film and Antibacterial Activity Test (The Study of Glycerol Concentration and Beluntas L. Industria: Jurnal Teknologi Dan Manajemen Agroindustri, 5(3), 149158. https://doi.org/https://doi.org/10.21776/ub.industria.2016.005.03.5
Nurhalimah, H., Wijayanti, N., & Widyaningsih, T. D. (2015). Efek Antidiare Ekstrak Daun Beluntas pada Mencit. Jurnal Pangan dan Agroindustri, 3(3), 10831094. https://jpa.ub.ac.id/index.php/jpa/article/view/231.
Pan, X., Chen, F., Wu, T., Tang, H., & Zhao, Z. (2009). The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Food Control, 20(6), 598602. https://doi.org/10.1016/j.foodcont.2008.08.019.
Paz, J.E.W., Contreras, C.R., Munguia, A.R., Aguilar, C.R., Inungaray, M.L.C. (2017). Phenolic content and antibacterial activity of extracts of Hamelia patensobtained by different extraction methods. Braz J Microbiol. 49(3), 656661.
Puspitasari, S. A., & Proyogo, L. S. (2017). Kadar Fenolik Total Ekstrak Etanol Daun Kersen (Muntingia calabura). Jurnal Ilmiah Cendekia Eksakta, 2(1), 18. https://doi.org/Perbandingan Metode Ekstraksi Maserasi Dan Sokletasi Terhadap Kadar Fenolik Total Ekstrak Etanol Daun Kersen (Muntingia calabura).
Prayoga, E. (2013). Perbandingan Efek Ektrak Daun Sirih Hijau (Piper betle L) Dengan Metode Difusi Disk dan Sumuran Terhadap Bakteri Staphylococcus aureu. Tesis. 1-3. Universitas Islam Negeri Syarif Hidayatullah Jakarta.
Syukur,R., Alam, G., Mufidah., & Rahim, R. T. (2011). Aktivitas Antiradikal Bebas Beberapa Ekstrak Tanaman Familia Fabaceae Radical. Kesehatan, 1(1), 6167. http://pasca.unhas.ac.id/jurnal/files/e72a6e54ecc12a1c6d06853e9e5cd778.pdf.
Radji and Maksum., (2011). Buku Ajar Mikrobiologi Panduan Mahasiswa Farmasi & Kedokteran. Jakarta: Kedokteran ECG.
Robinson, T. 1991. Kandungan Organik Tumbuhan Tingkat Tinggi. Diterjemahkan oleh Prof. Dr. Kosasih Padmawinata. Bandung: Penerbit ITB.
Amilah, S & Ajiningrum, P.S. (2015). Uji Efektivitas Daya Hambat Sari Daun Pegagan (Centella asiatica) dan Daun Beluntas (Pluchea indica Less) Terhadap Pertumbuhan Mycobacterium tuberculosis. Stigma Journal of Science, 8(2), 611. https://doi.org/https://doi.org/10.36456/stigma.vol8.no02.a253
Suru, E., Yamlean, P. V. Y., & Lolo, W. A. (2019). Formulasi dan Uji Efektivitas Krim Antibakteri Ekstrak Etanol Daun Beluntas (Pluchea indica Less .) terhadap Bakteri Propionibacterium acnes. PHARMACON, 8(2), 209218. https://doi.org/https://doi.org/10.35799/pha.8.2019.29256.
Thompson, E.B. (1985). Drug Biosreening. America: Graceway Publishing Company, Inc, Pp, 40, 118
Widyawati, P. S., WIjaya, C. H., Hardjosworo, P. S., & Sajuthi, D. (2011). Evaluasi Aktivitas Antioksidatif Ekstrak Daun Beluntas (Plucea indica) Berdasarkan Perbedaan Ruas Daun. Jurnal Teknologi Pangan, 5(1), 117. https://core.ac.uk/download/pdf/234615371.pdf.
Zein, U. (2004). Diare Akut Disebabkan Bakteri. E-USU Repository, January 2004, 115.https://www.researchgate.net/publication/42321299_Diare_Akut_Disebabkan_Bakteri.
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2020-09-29
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Lestari, K. A. P., Pranoto, P. P., Sofiyah, S., Musyirah, M., & Pratiwi, F. I. (2020). Antibacterial Activity of Beluntas (Pluchea indica L.) Leaves Extract using Different Extraction Methods. Jurnal Riset Biologi Dan Aplikasinya, 2(2), 49–54. https://doi.org/10.26740/jrba.v2n2.p49-54
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