Assessing Foliar Chlorophyll Content with SPAD-502 Chlorophyll Meter: A Comparison with Spectrophotometer Method in Various Plants

Authors

  • Siti Nurbaiti Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada Jalan Teknika Selatan, Sekip Utara, Yogyakarta, Indonesia, 55281
  • Asri Fajar Milasari Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada Jalan Teknika Selatan, Sekip Utara, Yogyakarta, Indonesia, 55281
  • Wiko Arif Wibowo Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada Jalan Teknika Selatan, Sekip Utara, Yogyakarta, Indonesia, 55281
  • Elvian Indah Nilamsari Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology Ikoma 630-0101, Japan
  • Diah Rachmawati Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada Jalan Teknika Selatan, Sekip Utara, Yogyakarta, Indonesia, 55281

DOI:

https://doi.org/10.26740/jrba.v7n1.p50-56

Keywords:

Chlorophyll analysis, Non-destructive measurement, Spectrophotometry; Leaf pigment

Abstract

Measuring chlorophyll content in plants is one of the main points that has never been ignored in various plant biology and agronomy research studies. Chlorophyll content is a parameter of growth and development, diagnosis of nutritional status, and response to environmental conditions. This study aimed to compare and determine the relationship between SPAD-502 chlorophyll meter readings and chlorophyll content obtained from acetone extraction followed by spectrophotometry measurement. Various leaves with different colors and thicknesses were used to determine the reliability of SPAD readings across diverse morphological traits. The results showed higher SPAD readings in leaves with a greener color. SPAD value was found to have a good linear relationship (r 0.8 and R2 0.64) and a positive correlation with total chlorophyll content, though variations due to leaf thickness suggest the need for correction factors. Furthermore, SPAD has potential as a rapid, non-destructive tool for monitoring plant health and nutrient status in agriculture, plant breeding, and horticulture. This tool can contribute to optimizing crop yield and managing fertilization practice, especially where the maintaining leaf integrity is essential for both commercial and aesthetic value. Species-specific calibration models are recommended to enhance measurement accuracy.

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Published

2025-03-31

How to Cite

Nurbaiti, S., Milasari, A. F., Wibowo, W. A., Nilamsari, E. I., & Rachmawati, D. (2025). Assessing Foliar Chlorophyll Content with SPAD-502 Chlorophyll Meter: A Comparison with Spectrophotometer Method in Various Plants. Jurnal Riset Biologi Dan Aplikasinya, 7(1), 50–56. https://doi.org/10.26740/jrba.v7n1.p50-56

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Vol. 7 No. 1 (2025)

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