Optimal Dose Determination of Gamma Ray Irradiation of Alocasia suhirmaniana Yuzammi & A. Hay: Optimal Dose Determination of Gamma Ray Irradiation of Alocasia suhirmaniana Yuzammi & A. Hay

Melza Mulyani; Iin Pertiwi A Husaini; Muhammad Rifqi Hariri; Rizmoon Zulkarnaen

doi:10.26740/jrba.v7n1.p31-37

Authors

Melza Mulyani Research Center for Applied Botany, National Research and Innovation Agency (BRIN), Jl. Raya Jakarta-Bogor Km 46, Cibinong, Bogor, West Java 16911, Indonesia
Iin Pertiwi A Husaini Research Center for Applied Botany, National Research and Innovation Agency (BRIN), Jl. Raya Jakarta-Bogor Km 46, Cibinong, Bogor, West Java 16911, Indonesia
Muhammad Rifqi Hariri Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Research Center for Applied Science - National Research and Innovation Agency, Jl. Raya Jakarta-Bogor Km 46, Cibinong, Bogor, West Java 16911, Indonesia
Rizmoon Zulkarnaen Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Research Center for Applied Science - National Research and Innovation Agency, Jl. Raya Jakarta-Bogor Km 46, Cibinong, Bogor, West Java 16911, Indonesia; Enviromental and Life Science Departement, Faculty of Science, Universiti Brunei Darussalam, Jl Tungku Link, BE 1410, Brunei Darussalam

DOI:

https://doi.org/10.26740/jrba.v7n1.p31-37

Keywords:

Alocasia suhirmaniana, Araceae, gamma ray, lethal dose, mutation

Abstract

Alocasia suhirmaniana Yuzammi & A. Hay belongs to the Araceae family and has the potential for ornamental use. Nevertheless, this plant exhibits a limited leaf count and pliable leaf stems, necessitating the application of breeding techniques for its improvement. One important strategy empeloyed to improve the quality of ornamental plants is the breeding, which involves the use of gamma ray irradiation techniques. The objective of this study is to determine the developmental patterns and appropriate dosage levels of LD20 and LD50 Gamma ray irradiation in A. suhirmaniana callus. The development of callus, the quantity of shoots, and the establishment of rooted explants exhibit a stochastic pattern, with a decrease observed at a dosage of 5 Gy and an increase observed at 10 Gy. The LD20 and LD50 values for Gamma ray irradiation in A. suhirmaniana callus fall within the dose range of 7.14 Gy and 15 Gy, respectively. This dosage range is recommendedto achieve a greater diversity in genotypes and phenotypes. By successfully finding the optimal dosage of gamma ray irradiation, plant selection can be improved through enhancing genotype and phenotype characteristics.

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Optimal Dose Determination of Gamma Ray Irradiation of Alocasia suhirmaniana Yuzammi & A. Hay