The Effect of Water Concentration on Growth Media on Lipid Production by Oleaginous Fungi Isolate BR 2.2

Herin Yoga Lesti; Miftahul Ilmi

doi:10.26740/jrba.v4n2.p51-56

Authors

Herin Yoga Lesti Faculty of Biology, Universitas Gadjah Mada
Miftahul Ilmi Faculty of Biology, Universitas Gadjah Mada https://orcid.org/0000-0001-5112-9865

DOI:

https://doi.org/10.26740/jrba.v4n2.p51-56

Keywords:

Oleaginous fungi, glucose, water content, lipid, biomass

Abstract

Oleaginous fungi are one of the microorganisms that can accumulate a high number of biomasses quickly (within 96-130 hours) and are often used to produce lipids. The growth of fungi depends on the chemical composition of the environment in which it grows. The growth media of fungi must contain high carbohydrates as a source of nutrients and high nitrogen content. One of the carbon sources that fungi can use in the growth process is glucose. BR 2.2 isolate is an oleaginous fungus capable of accumulating 28.44% lipids from the total dry biomass with glucose as a carbon source in 50 mL of growth media. Therefore, this study was conducted to determine the effect of variations in the volume of media and incubation time on the production of biomass and lipid isolate BR 2.2. Biomass and lipid production were analyzed at media with additional water volumes of 10, 20, 30, 40, and 50 mL with 48, 96, and 144 hours of incubation times. The results showed that lipid accumulation and biomass production increased with the reduction of water content in the growth media and reached the highest number in the media volume of 20 mL with an incubation time of 144 hours, i.e., 0.87±0.04 g/L and 12.53±0.29 g/L. It can be concluded that biomass and fungal lipid increased along with incubation time and nutrient concentration.

References

Asadollahzadeh, M., Ghasemian, A., Saraeian, A., Resalati, H., & Taherzadeh, M. J. (2018). Production of fungal biomass protein by filamentous fungi cultivation on liquid waste streams from pulping process, BioRes, 13(3), 5013-5031. https://bioresources.cnr.ncsu.edu/resources/production-of-fungal-biomass-protein-by-filamentous-fungi-cultivation-on-liquid-waste-streams-from-pulping-process/

Axelsson, M., & Gentili, F. (2014). A single-step method for rapid extraction of total lipids from green microalgae. PLoS ONE, 9(2), e89643. https://doi.org/10.1371/journal.pone.0089643

Barboráková, Z., Labuda, R., Häubl, G., & Tančinová, D. (2012). Effect of glucose concentration and growth conditions on the fungal biomass, ph of media and production of fumagillin by a non-pathogenic strain penicillium scabrosum. Journal of Microbiology, 4, 466–477.https://www.jmbfs.org/wpcontent/uploads/2012/01/Barborakova.pdf

Basu, S., Bose, C., Ojha, N., Das, N., Das, J., Pal, M., & Khurana, S. (2015). Evolution of bacterial and fungal growth media. Bioinformation, 11(4),182. https://doi.org/10.6026%2F97320630011182

Daly, P., Peng, M., Mitchell, H. D., Kim, Y. M., Ansong, C., Brewer, H., de Gijsel, P., Lipton, M. S., Markillie, L. M., Nicora, C. D., Orr, G., Wiebenga, A., Hildén, K. S., Kabel, M. A., Baker, S. E., Mäkelä, M. R., & de Vries, R. P. (2020). Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation. Environmental Microbiology, 22(3), 1154–1166. https://doi.org/10.1111/1462-2920.14907

Dey, P., Mall, N., Chattopadhyay, A., Chakraborty, M., & Maiti, M. K. (2014). Enhancement of lipid productivity in oleaginous colletotrichum fungus through genetic transformation using the yeast ctDGAT2b gene under model-optimized growth condition. PLoS ONE, 9(11), e0187171. https://doi.org/10.1371/journal.pone.0111253

Doriya, K., Jose, N., Gowda, M., & Kumar, D. S. (2016). Solid-State Fermentation vs Submerged Fermentation for the Production of L-Asparaginase. Advances in Food and Nutrition Research, 78, 115–135. https://doi.org/10.1016/bs.afnr.2016.05.003

Fifendy, M. (2017). Mikrobiologi. Depok: Kencana

Fuentes, M. E., Quiñones, R. A., Gutiérrez, M. H., & Pantoja, S. (2015). Effects of temperature and glucose concentration on the growth and respiration of fungal species isolated from a highly productive coastal upwelling ecosystem. Fungal Ecology, 13, 135–149. https://doi.org/10.1016/j.funeco.2014.09.006

Gohel, H., Ghosh, S., & Braganza, V. (2013). Yeast as a Viable and Prolonged Feedstock for Biodiesel Production. Article in International Journal of Renewable Energy Research, 3(1), 126–131. https://doi.org/10.20508/ijrer.v3i1.488.g6115

Hosseinpour, L., Zareei, M., Borjian Boroujeni, Z., Yaghoubi, R., Jamal Hashemi, S., & Author, C. (2017). Effect of Different Incubation Temperatures, Times, and Colored Lights on Fungal Biomass and Black Pigment (Melanin) Production in Exophiala crusticola. Infect Epidemiol Microbiol, 3(3), 90–95. https://doi.org/10.18869/modares.iem.3.3.90

Manpreet, S., Sawraj, S., Sachin, D., & Banerjee, S. (2005). Influence of Process Parameters on the Production of Metabolites in Solid-State Fermentation. Malalaysian Jounal of Microbiology, 1(2), 1–9. https://agris.fao.org/agris-search/search.do?recordID=DJ2021067075

Meeuwse, P. (2011). Production of fungal lipids Kinetic modeling and process design. The Netherlands

Mienda, B., & Idi, A. (2011). Microbiological Features of Solid-State Fermentation and its Applications. An overview Model-Guided Metabolic Gene Knockouts Strategies for Enhanced Succinic Acid Production in Escherichia coli Strain K-12 View project. Research in Biotechnology, 2(6), 21–26. https://www.researchgate.net/publication/236177189

Murwani, S. (2015). Dasar-dasar Mikrobiologi Veteriner. Malang: UB Press. pp 151-151

Rizki, M. A. A. H., & Ilmi, M. (2021). The Potential of Oleaginous Filamentous Fungi Isolated from Soil of Baturraden Botanical Garden, Central Java, Indonesia. IOP Conference Series: Earth and Environmental Science, 736(1). https://doi.org/10.1088/1755-1315/736/1/012060

Sergeeva, Y. E., Galanina, L. A., Andrianova, D. A., & Feofilova, E. P. (2008). Lipids of filamentous fungi as a material for producing biodiesel fuel. Applied Biochemistry and Microbiology, 44(5), 523–527. https://doi.org/10.1134/S0003683808050128

Shafiq, S. A., & Chechan, R. A. (2019). Influence of different natural media on production of myco-diesel. IOP Conference Series: Earth and Environmental Science, 388(1). https://doi.org/10.1088/1755-1315/388/1/012054

Somashekar, D., Venkateshwaran, G., Sambaiah, K., & Lokesh, B. R. (2002). Effect of culture conditions on lipid and gamma-linolenic acid production by mucoraceous fungi. Process Biochemistry, 38(12), 1719–1724. https://doi.org/10.1016/S0032-9592(02)00258-3

Vazquez-Duhalt, R., & Greppin, H. (1987). Growth and production of cell constituents in batch cultures of botryococcus sudeticus. Pergamon Journals, 26(4), 885–889. https://doi.org/10.1016/S0031-9422(00)82311-0

Wu, S., Hu, C., Jin, G., Zhao, X., & Zhao, Z. K. (2010). Phosphate-limitation mediated lipid production by Rhodosporidium toruloides. Bioresource Technology, 101(15), 6124–6129. https://doi.org/10.1016/j.biortech.2010.02.111

Yang, Y., Heidari, F., & Hu, B. (2019). Fungi (mold)-based lipid production. Methods in Molecular Biology, 1995, 51–89. https://doi.org/10.1007/978-1-4939-9484-7_3.