Antibacterial Activity of Garlic Added to Tempeh against Bacillus sp. and Escherichia coli

Authors

  • Adelia Prasetya Faculty of Biology, Satya Wacana Christian University, Jl. Diponegoro 52-60 Salatiga - Indonesia
  • Lusiawati Dewi Faculty of Biology, Satya Wacana Christian University, Jl. Diponegoro 52-60 Salatiga - Indonesia
  • Risya Pramana Situmorang Faculty of Biology, Satya Wacana Christian University, Jl. Diponegoro 52-60 Salatiga - Indonesia

DOI:

https://doi.org/10.26740/jrba.v4n1.p34-41

Keywords:

antibacterial activity, fermentation, garlic, tempe

Abstract

Tempeh (tempe) has been known to have antibacterial properties due to the presence of glycoprotein compounds, antimicrobial peptides, and flavonoids produced by the fungus Rhizopus spp. during the fermentation process. The addition of garlic powder is expected to increase the antibacterial activity of tempeh against contaminant bacteria. This research aimed to analyze the antibacterial activity, water content, and organoleptic properties (taste, aroma, and color) of tempeh with garlic powder supplementation. Garlic powder was added to the tempeh fermentation process with various concentrations of 0.15, 0.25, 0.50, and 0.75%. Antibacterial activity, water content, and qualitative organoleptic tests were analyzed using paper disk diffusion, gravimetric, and questionnaire methods. The results showed that the addition of garlic powder in all treatments for Bacillus sp had shown an inhibitory activity with moderate strength with an inhibitory power of 0.15; 0.25; 0.50; and 0.75% respectively 8±1.71; 10±1.71; 7±0.96; 9±0.58. Meanwhile, Escherichia coli showed no inhibitory activity. The addition of garlic powder at a concentration of 0.25% reduced the water content to 58.25±0.03. In addition, the addition of garlic powder was not able to affect the color but was able to influence the aroma and taste of tempeh. Garlic powder was able to inhibit Bacillus sp with the best concentration of 0.25%; the addition of garlic powder can reduce the water content and affect the aroma and taste of tempeh.

References

Badan Standardisasi Nasional. 2015. Tempe Kedelai. In Tempe Kedelai SNI 344 : 2015. Badan Standardisasi Nasional, Jakarta

Afriani, N., Yusmarini, & Pato, U. (2017). Aktivitas Antimikroba Lactobacillus plantarum 1 yang Diisolasi dari Industri Pengolahan Pati Sagu terhadap Bakteri Patogen Escherichia coli FNCC-19 dan Staphylococcus aureus FNCC-15. Jom Faperta, 4(2), 1–12

Banobe, C. ., Kusumawati, I. G. A. W., & Wiradnyani, N. . (2019). Nilai Zat Gizi Makro dan Aktivitas Antioksidan Tempe Kedelai (Glycine maz L.) Kombinasi Biji Kecipir (Psophocarpus tetragonolobus L.). Pro Food (Jurnal Ilmu Dan Teknologi Pangan, 5(2), 486–495

Barus, T., Salim, D. P., & Hartanti, A. T. (2019). Kualitas Tempe menggunakan Rhizopus delemar TB 26 dan R. delemar TB 37 yang Diisolasi dari Inokulum Tradisional Tempe “daun waru.” Jurnal Aplikasi Teknologi Pangan, 8(4), 143–148

Budiono, R. A. (2016). Pengaruh Jenis Kapang terhadap Aktivitas Fermentasi Tempe Saga Pohon (Adenanthera pavonina L.) [Skripsi]. Jakarta: Fakultas Sains dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah

Kemit, N., Widarta, I. W. R., & Nocianitri, K. A. (2016). Pengaruh Jenis Pelarut dan Waktu Maserasi terhadap Kandungan Senyawa Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Alpukat (Persea Americana Mill). Jurnal Ilmu Dan Teknologi Pangan, 5(2), 130–141

Laksono, A. S., Marniza, & Rosalina, Y. (2019). Karakteristik Mutu Tempe Kedelai Lokal Varietas Anjasmoro dengan Variasi Lama Perebusan dan Penggunaan Jenis Pengemas. Jurnal Agroindustri, 9(1), 8–18

Lestari, S. R., Witjoro, A., & Poejiani, S. (2018). The Potential of Single Garlic Oil in Inhibiting The Growth and Damaging The Membrane of Pseudomonas aeruginosa Bacteria. Journal of Tropical Biodiversity and Biotechnology, 3(3), 67–72. https://doi.org/10.22146/jtbb.33498

Lisiswanti, R., & Haryanto, F. P. (2017). Allicin pada Bawang Putih ( Allium sativum ) sebagai Terapi Alternatif Diabetes Melitus Tipe 2. Majority, 6(2), 31–36

Mawaddah, N., Fakhrurrazi, & Rosmaidar. (2018). Aktivitas Antibakteri Ekstrak Tempe Terhadap Bakteri Staphylococcus aureus. Jurnal Ilmiah Mahasiswa Veteran, 2(3), 230–241

Moulia, M. N., Syarief, R., Iriani, E. S., & Kusumaningrum, H. D. (2018). Antimikroba Ekstrak Bawang Putih. Jurnal Pangan, 27(1), 55–66

Noviana, A., Dieny, F. F., Rustanti, N., Anjani, G., & Afifah, D. N. (2018). Antimicrobial Activity of Tempeh Gembus Hydrolyzate. IOP Conference Series Earth and Environmental Science, 116(1), 1–7.

Pratama, M. (2017). Identifikasi Atribut dan Rasa Rempah dengan Profiled Test. Jurnal Agroindustri Halal, 3(2), 126–132

Pratiwi, R. H. (2017). Mekanisme Pertahanan Bakteri Patogen terhadap Antibiotik. Jurnal Pro-Life, 4(3), 418–429

Purwantiningsih, T. I., Rusae, A., & Freitas, Z. (2019). Uji In Vitro Antibakteri Ekstrak Bawang Putih sebagai Bahan Alami untuk Menghambat Bakteri Staphylococcus aureus dan Escherichia coli. Sains Peternakan, 17(1), 1–4

Purwanto, Y. A., & Weliana. (2018). Kualitas Tempe Kedelai pada Berbagai Suhu Penyimpanan. Journal of Agro-Based Industry, 35(2), 106–112

Rahman, M. S. (2014). Allicin and Other Functional Active Components in Garlic : Health Benefits and Bioavailibility. International Journal of Food Properties, 10(2), 245–268

Rahmi, S. L., Mursyid, & Wulansari, D. (2018). Formulasi Tempe Berbumbu serta Pengujian Kandungan Gizi. Industria : Jurnal Teknologi Dan Manajemen Agroindustri, 7(1), 57–65

Salima, J. (2015). Antibacterial Activity of Garlic (Allium sativum l.). MAJORITY, 4(2), 30–39

Soraya, C., Chismirina, S., & Novita, R. (2018). Pengaruh Perasan Bawang Putih (Allium sativum L.) sebagai Bahan Irigasi Saluran Akar Dalam Menghambat Pertumbuhan Enterococcus faecalis Secara In Vitro. Cakradonya Dental Journal, 10(1), 1–9

Sudjatini. (2020). Pengaruh Cara Pengolahan terhadap Aktivitas Antioksidan Ekstrak Bawang Putih (Allium sativum L.) Varietas Kating dan Sinco. Agrotech Jurnal Ilmiah Teknologi Pertanian, 3(1), 1–7

Wiryadana, K. A., Pinatih, K. J. P., & Hendrayana, M. A. (2019). Uji Daya Hambat Kombinasi Siproflaksasin dengan Obat Non - Antibiotik Artesunat, Diklofenak dan Loperamid terhadap Pertumbuhan Isolat Klinis Escherichia coli. E-Jurnal Medika, 8(3), 1–8

Zhafira, R. (2018). Pengaruh Lama Aging terhadap Sifat Fisik, Kimia, dan Aktivitas Antioksidan Produk Bawang Hitam Lanang. Jurnal Pangan Dan Agroindustri, 6(1), 34–42.

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Published

2022-03-31

How to Cite

Prasetya, A., Dewi, L. ., & Pramana Situmorang, R. . (2022). Antibacterial Activity of Garlic Added to Tempeh against Bacillus sp. and Escherichia coli . Jurnal Riset Biologi Dan Aplikasinya, 4(1), 34–41. https://doi.org/10.26740/jrba.v4n1.p34-41

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