Screening of Extracellular Enzymes on Serratia marcescens strain MBC1

Authors

  • Meishy Handerlin Putri Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia
  • Kusuma Handayani Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia
  • Wawan A Setiawan Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia
  • Berliana Damayanti Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia
  • Cindy Lukyta Ratih Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia
  • Achmad Arifiyanto Biology Departement, Faculty of Mathematics and Natural Sciences, Lampung University Jln. Prof Soemantri Brojonegoro 1, Rajabasa District, Bandar Lampung, Lampung 35144, Indonesia

DOI:

https://doi.org/10.26740/jrba.v3n1.p23-29

Keywords:

Hydrolase enzyme, Serratia marcescens strain MBC1, enzymatic activity

Abstract

Hydrolase enzymes are a class of enzymes used to break down the organic substrates into simpler molecules. Hydrolase enzymes play a role in biocontrol, industry, and public health. One of the hydrolase enzyme producers is bacteria. Serratia marcescens strain MBC1 is a collection in the Microbiology Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung which enzymatic activities havent been known yet. This research aimed to determine the enzymatic activity of S. marcesescens strain MBC1 that can be used as a candidate for biocontrol agents, biosurfactant producers, industry, or in the health sector. The screening was carried out using a qualitative method plate assay using selective solid media for each test, with three replications for each test. The data obtained were analyzed statistically using analysis non-parametric kruskall-wallis test. The lipolytic activity of the S. marcescens strain MBC1 showed high efficacy with an enzymatic index of 5.52 and amylase, cellulases, protease, mannanase, and chitinase with enzymatic indexes, respectively, 4.17, 1.50, 0.69, 0.35, and 0.27. This enzyme activity of this isolate can be used as a candidate for biological control agents,biosurfactant producers, industry, or the health sector.


References

Arfah, R. A., Lestari, Y. A., Dali, S., Muliadi, & Liestianty, D. (2020). The Isolation of Termofil Amilolitik Bacterium and Activity Test of Harsh Extract Amylase Enzyme from the Hot Spring at Jailolo Bay in North Maluku. Journal of Physics: Conference Series, 1569(4). https://doi.org/10.1088/1742-6596/1569/4/042050.

Arifiyanto, A., Apriyanti, F. D., Purwaningsih, P., Kalqutny, S. H., Agustina, D., Surtiningsih, T., Shovitri, M., & Zulaika, E. (2017). Lead (Pb) Bioaccumulation; Genera Bacillus Isolate S1 and SS19 as a Case Study. AIP Conference Proceedings, 1854(1). https://doi.org/10.1063/1.4985394.

Arifiyanto, A., Hapin, A., Meishy Handerlin, P., Berliana, D., & Cindy, L. R. (2021). The biological prospective of red-pigmented bacteria cultured from contaminated agar media. Biodiversitas, 22(3), 11521159. https://doi.org/10.13057/biodiv/d220310.

Artha, O. A., Sudarno, H, P., & L. A, S. (2019). Identification of Extracellular Enzyme-Producing Bacteria (Proteolytic, Cellulolytic, and Amylolytic) In The Sediment Of Extensive Ponds In Tanggulrejo, Gresik. IOP Conference Series: Earth and Environmental Science, 236(1). https://doi.org/10.1088/1755-1315/236/1/012003.

Cahyani, P., Wijanarka, & Budi, R. (2017). Aktivitas Spesifik Selulase Serratia Marcescens Dengan Variasi Konsentrasi Amonium Sulfat ((Nh4)2So4) dan Ph. Jurnal Biologi, 6(2), 4149. Retrieved from https://ejournal3.undip.ac.id/index.php/biologi/article/view/19535.

Ervina, E., Ekowati, C. N., Sumardi, & Emantis, R. (2020). Lipolytic-screening of Bacillus genera as Biocontrol candidate In Coffee Plantation. Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati, 7(1), 3134. http://dx.doi.org/10.23960%2Fj_bekh.v7i1.2496.

Halimah, N., Baktir, & Purkan, P. (2019). Exploration of Cellulolytic Microorganism as A Biocatalyst Candidate for Liquid Fertilizer Production. IOP Conference Series: Earth and Environmental Science, 217(1). Retrieved from https://iopscience.iop.org/article/10.1088/1755-1315/217/1/012021/meta.

Irdawati, Putri, N. D., Syamsuardi, Agustien, A., & Rilda, Y. (2020). Potential of Xylanase Thermophilic Bacteria in the Pulp Biobleaching Process. ICoBioSE 2019, 10, 2327. https://doi.org/10.2991/absr.k.200807.006.

Istianah, D., Ulfah, U., & Ahmad, B. (2019). Karakterisasi Enzim Amilase dari Bakteri Bacillus megaterium pada Variasi Suhu, pH dan Konsentrasi Substrat. Jurnal Riset Biologi dan Aplikasinya, 1(2), 1825. http://dx.doi.org/10.26740/jrba.v2n1.p11-17.

Kahrarian, Z., Saeed, K., & Ahmad, T. (2019). A Review of the Production of Biosurfactant and Pigmented Prodigyosin by Bacteria Serrassia marsseñes. Asian Journal of Research in Medicine and Medical Science, 1(1), 1824.

Linda, T. M., Siregar, S., Fitri, W. D., Martina, A., Lestari, W., Roslim, D. I., & Hapsoh. (2018). Isolation and screening of culturable endophytic bacteria from leaf of rubber plant that produces of chitinase. Journal of Physics: Conference Series, 1116(5). https://doi.org/10.1088/17426596/1116/5/052038.

Mohanasrinivasan, V., C. Subathra, D., D., J., E., S., & S. Jemimah, N. (2018). Purification and Characterization of Extracellular Lipase from Serratia marcescens VITSD2. Proceedings of the National Academy of Sciences India Section B - Biological Sciences, 88(1), 373381. https://doi.org/10.1007/s40011-016-0763-6.

Mohanram, R., Murlidhar, J. M., & Susan, T. (2020). Isolation, Identification and Molecular Characterization of Serratia Marcescens Strain Nmrl65 an Opportunistic Human Pathogen From the Spoiled Coconut. International Journal of Environment and Health Sciences, 2(2), 8186. https://doi.org/10.47062/1190.0202.02.

Okay, S., Özdal, M., & Kurbanoǧlu, E. B. (2013). Characterization, Antifungal activity, and Cell immobilization of a Chitinase from Serratia marcescens MO-1. Turkish Journal of Biology, 37(6), 639644. https://doi.org/10.3906/biy-1208-45.

Olaniyi, O., & Arotupin, D. (2013). Isolation and Screening of Mannanase Producing Bacteria from Agricultural Wastes. British Microbiology Research Journal, 3(4), 654663.https://doi.org/10.9734/bmrj/2013/4221.

Padmavathi, T., Rayavarapu, B., Purushothama, R. P., Niranjan, N. R., & Pavitra, P. V. (2018). Screening of Potential Probiotic Lactic Acid Bacteria And Production of Amylase and Its Partial Purification. Journal of Genetic Engineering and Biotechnology, 16(2), 357362. https://doi.org/10.1016/j.jgeb.2018.03.005.

Pratika, M., M, A., & Suwastika. (2021). Protease Activity From Bacterial Isolates of Nepenthes maxima reinw. ex nees. Journal of Physics: Conference Series, 1763(1), 012092.https://doi.org/10.1088/17426596/1763/1/012092.

Puspadewi, R., Putranti, A., Mira, A., & Helma, K. (2018). Aktivitas Ekstraseluler yang Berasal dari Cladosporium tenuissimum. Seminar Nasional Farmasi (SNIFA) 4 UNJANI, 125 132.

Rosa, E., C.N, E., Tundjung Tripeni, H., Ahmad, I., Ferly, A., & Achmad, A. (2020). Characterization of Entomopathogenic Fungi as A Natural Biological Control Of American Cockroaches (Periplaneta americana). Biodiversitas, 21(11), 52765282. https://doi.org/10.13057/biodiv/d211131.

Sumardi. (2005). Optimasi Produksi Enzim β -Mananase Ekstraseluler dari Bakteri Geobacillus stearothermophilus L-07. Jurnal Sains Teknologi, 11(2), 6671.

Susilowati, D. N., Indah, S., Kristina Dwi, A., & Erny, Y. (2018). Penapisan Kapang Asal Lahan Sulfat Masam Kalimantan Selatan Sebagai Penghasil Enzim Ekstraseluler. Ilmu Pertanian, 1(21), 6582. https://doi.org/10.24246/agric.2020.v32.i1.p65-82.

Tubkanlu, E., Aminzadeh, Karkhane, Alikhajeh, & Ghoroghi. (2019). Serratia marcescens B4A Chitinase Thermostability Enhancement by S390I QuikChange Site Directed Mutagenesis. Iranian Journal of Fisheries Sciences, 18(4), 10461059. https://doi.org/10.22092/ijfs.2019.119292.

Wardoyo, F. A., & Aprilia, I. K. (2018). Peningkatan Stabilitas Termal dan Stabilitas Penggunaan Berulang Enzim Lipase Melalui Imobilisasi Pada Zeolit Alam. Jurnal Labora Medika, 2(1), 15. Retrieved from https://jurnal.unimus.ac.id/index.php/JLabMed.

Wijaya, P. S., Sri, W & Nur, A. (2017). Morfologi dan Karakterisasi Pertumbuhan Bakteri Asam Laktat (um 1.4a) dari Proses Fermentasi Wikau Maombo Untuk Studi Awal Produksi Enzim Amilase. Jurnal Sains dan Teknologi Pangan, 2(4), 657-663. http://dx.doi.org/10.33772/jstp.v2i5.3745.

Downloads

Published

2021-03-31

How to Cite

Putri, M. H., Handayani, K., Setiawan, W. A., Damayanti, B., Ratih, C. L., & Arifiyanto, A. (2021). Screening of Extracellular Enzymes on Serratia marcescens strain MBC1. Jurnal Riset Biologi Dan Aplikasinya, 3(1), 23–29. https://doi.org/10.26740/jrba.v3n1.p23-29

Issue

Section

Articles
Abstract views: 589 , PDF Downloads: 499