Isolation and Characterization of Cellulolytic Bacteria from Senduro Goat Feces, Lumajang
DOI:
https://doi.org/10.26740/lenterabio.v15n2.p167-174Kata Kunci:
Cellulolytic bacteria, goat feces, cellulolytic index, characterization, biodiversityAbstrak
Cellulolytic bacteria are bacteria that can produce cellulase enzymes. Goat feces can serve as a source for various types of cellulolytic bacteria isolates with diverse cellulose-degrading abilities. The purpose of this study is to isolate and characterize cellulolytic bacterial isolates in Senduro goat feces. This research was an observational analytic study involving isolation using CMC media, testing cellulolytic activity using Congo red, and characterization (including colony morphological characteristics, cell characteristics, and biochemical characteristics) of the cellulolytic bacterial isolates with the highest cellulolytic index. Based on the results, 18 cellulolytic bacterial isolates with varying levels of cellulolytic activity were obtained. Four isolates (BSD5, BSD7, BSA2, and BSA8) was found to have the highest cellulolytic index values of 3.1, 2.9, 2.2, and 2.2, respectively. In conclusion, evaluation of colony morphology, cellular characteristics, and biochemical profiles indicated that isolate BSD5 showed similarity to the genus Bacillus, BSD7 to Alcaligenes, BSA2 to Enterococcus, and BSA8 to Cellulomonas.
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