UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BELUNTAS (Pluchea indica L.) DENGAN METODE DPPH (2, 2-DIFENIL-1-PIKRILHIDRAZIL)
DOI:
https://doi.org/10.26740/icaj.v2n2.p25-28Keywords:
Antioxidant activity, DPPH, beluntas leaves, ethanol extractAbstract
Antioxidant is chemical compound that can give one or more electron to free radical which can obstruct free radical reaction. Beluntas (Pluchea indica L.) is one of plant which potentially as antioxidant. The aim of this research is to determine the antioxidant activity of etanol extract of beluntas leaves. Antioxidant activity test was done by using DPPH (2, 2-diphenyl-1- picrylhydrazil) method. The dry powder of beluntass leaves was macerated by using ethanol. Ethanol extract obtained was tested antioxidant activity to get IC-50 value by using UV-Vis spectrophotometry at 517 nm. Antioxidant activity test results showed that ethanol extract of beluntass leaves had IC50 value of 37,25 ppm. So it had antioxidant activity in strong category.References
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H. œIdentifikasi senyawa flavonoid dan kapasitas antioksidannya pada ekstrak sayuran indigenous Jawa Barat, Half Day Seminar on Natural Antioxidants: Chemistry, Biochemistry and Technology; 2008.
[2] Anese M, Lara M, Maria CN, œAntioxidant Properties of Tomato Juice As Accected By Heating, J. of The Sci. of Food and Agri., 1999.
[3] Agoes A. 2010. Tanaman Obat Indonesia. Airlangga; Jakarta.
[4] Pujowati P, Pengenalan Ragam Tanaman Lanskap Asteraceae. Bogor: Institut Pertanian Bogor, 2006.
[5] Dean, J. Extraction Techniques In Analytical Science. London: John Wiley And Sons LTD. 2009.
[6] Badarinath A, Rao K, Chetty CS, Ramkanth S, Rajan T, & Gnanaprakash K. œA Review on In-vitro Antioxidant Methods: Comparisons, Correlations, and Considerations. International Journal of PharmTech Research, 1276-1285. 2010.
[7] Ardiansyah. Nuraida, L. Andarwulan, œAktivitas antimikroba daun beluntas (Pluchea indica Less) dan stabilitas aktivitasnya pada berbagai konsentrasi garam dan tingkat Ph. Jurnal Teknologi dan Industri Pangan., 14(2), 90-97. 2003.
[8] Zou, Y., Lu, Y., dan Wei, D., œAntioxidant Activity of a Flavonoid-Rich Extract of Hypericum perforatum L. In Vitro. J. Agric. Food Chem, 52(1), 5032-5039
[9] Luger, P. Weber, M. Dung, N.X.Ngoc, P.H., Tuong, D.T. dan Rang, D.D., œThe crystal structure of hop- 17(21)-en-3-yl asetat of Plucheapteropoda Hemsl, Crystal Res Technology 35(3), 355-362. 2002.
[10] Biswas, R. Dasgupta, A. Mitra, A. Roy, S.K. Dutta, P.K. Achari, B. Dastidar, S.G. dan Chatterjee, œIsolation, purification and characterization of four pure compounds from the root extract of Pluchea indica Less and the potentiality of the root extract and the pure compounds for antimicrobial activity. European Bulletin of Drug Research, 13 (6370), 2005.
[11] Marxen K, Vanselow KH, Lippemeier S, Hintze R., œDetermination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements. Sensors. 2007.
[12] Prayoga G. Fraksinasi, œUji Aktivitas Antioksidan dengan Metode DPPH dan Identifikasi Golongan Senyawa Kimia dari Ekstrak Teraktif Daun Sambang Darah (Excoecaria cochinchinensis Lour). Fakultas Farmasi Program Studi Sarjana Ekstensi Universitas Indonesia. 2013.
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2019-03-19
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