LenteraBio : Berkala Ilmiah Biologi

Antibacterial Activity of Young Papaya Fruit Extract (Carica papaya L.) Against Acne-inducing Propionibacterium acnes

Dhesy Dwi Retno Purwandhini — 2024-06-21

Acne is one of the skin disorders that often attracts the attention of teenagers and young adults. The inappropriate use of antibiotics in the treatment of acne can lead to resistance, thus requiring alternatives from natural ingredients that have the ability as antibacterials. This study was conducted to test the antibacterial properties of young papaya fruit extract against the growth of P. acnes and determine the optimal concentration of young papaya fruit extract in stopping the growth of P. acnes. Antibacterial testing used the pitting method with a completely randomized design (CRD). Data analysis using Kolmogorov-Smirnov test, One Way Anova test, and Duncan test showed that the treatment of young papaya fruit extract concentration had a positive effect on the growth of P. acnes and there were significant differences with other treatments. The results of testing the antibacterial activity of young papaya fruit extract with a concentration of 20%, 40%, and 60% produced an average inhibition zone sequentially of 4,60 ± 0,54 mm, 7,60 ± 0,54 mm, and 10,80 ± 0,83 mm so that the 60% concentration can provide optimal results in stopping the growth of P. acnes because it forms the largest inhibition zone. Thus, this study proves that young papaya fruit extract has antibacterial properties so that it can be applied as a natural acne medicine.

Isolation and Evaluation of Antifungal Activity of Sand Sea Cucumber Symbiont Bacteria (Holothuria scabra) from Wakatobi National Park against Malassezia furfur

Nur Arfa Yanti — 2024-05-29

Malassezia furfur is a fungus that causes tinea versicolor which requires antifungal compounds for treatment. Antifungal compounds can be obtained from bacteria that are in symbiosis with marine organisms. One candidate for bacteria producing antifungal compounds is the sand sea cucumber symbiont bacteria (Holothuria scabra). This research aims to obtain bacterial isolates that are able to inhibit the growth of the Malassezia furfur fungus that causes tinea versicolor (Pityriasis versicolor). Isolation of sea cucumber symbiont bacteria was carried out using the pour plate method. The antifungal activity of symbiont bacteria was carried out using the well diffusion method. The results of the research obtained 5 isolates of the sand sea cucumber symbiont bacteria which had M. furfur antifungal activity. Three bacterial isolates, namely isolates DT1, DT4 and isolate DT5, produced antifungal compounds which were able to inhibit the growth of M. furfur with an inhibition zone ranging from 8.60 mm-11.30 mm within 24 hours. Based on the identification results using the profile matching method, it is known that the bacterial isolates DT1 and DT5 belong to the genus Pseudomonas, while the bacterial isolate DT4 belongs to the genus Bacillus. Therefore, the three isolates of sand sea cucumber symbiont bacteria can be developed and applied to produce medicinal ingredients for tinea versicolor skin disease.

Potential Source of Tree Seeds at Jering Menduyung Nature Tourism Park, West Bangka, Bangka Belitung Islands

Yunita Lestari — 2024-06-21

Jering Menduyung Nature Tourism Park (NTP) still has relatively well-preserved natural areas. This area is known as Keruing Forest because of the large number of keruing hitam stands with large diameter. This study aims to identify the species that have potential to serve as seed sources in this area. Vegetation data and microclimate measurements were carried out in 21, 20 x 20 m square plots (=0.84 ha). Identification was carried out by matching specimens with the collection of Herbarium Bangka Belitungense. The species found were analyzed in reference to the Regulation of the Minister of Environment and Forestry of the Republic of Indonesia Number P.3/MENLHK/SETJEN/KUM.1/1/2020 regarding the Implementation of Forest Plant Seeds. The results show that pules (Guioa pubescens (Zoll. & Moritzi) Radlk.) and keruing hitam (Dipterocarpus grandiflorus (Blanco) Blanco) can be used as source of identifiable seeds stands. Both species have seedlings that grow in clusters near their parent tree. However, due to insufficient spacing between parent trees, they do not meet the utilization requirements outlined in the SNI 8806:2019 Source of forest tree propagules, these two species cannot be used as natural seed sources. Vegetative propagation or a policy review can be considered as alternative solutions.

Antibacterial Activity of Ethanol Extract of Thorn Spinach Leaves (Amaranthus spinosus L.) against Streptococcus mutans

Ria Dwi Setiani — 2024-06-21

Dental caries is a disease that attacks oral health that many people suffer from. Dental caries is caused by the activity of the bacteria Streptococcus mutans. Thorn spinach (Amaranthus spinosus L.) is a medicinal plant that has secondary metabolite compounds that can have antibacterial properties. This research aims to determine the antibacterial activity and optimal concentration in inhibiting the growth of S. mutans. The stages of this research include preparation, extraction, making sample solutions, phytochemical screening, rejuvenation and making bacterial suspensions, as well as antibacterial activity tests. The antibacterial activity test used extract concentrations of 20%, 40%, 60% and 80%. These extract concentrations produced an average diameter of the inhibition zone 0.25 ± 0.50 mm; 1.50 ± 2.38 mm; 5.13 ± 0.75 mm; and 6.38 ± 0.25 mm. Data were analyzed using the Kolmogorov-Smirnov test, Duncan test, and Anova test. Based on the analysis data, it was concluded that there was antibacterial activity at each extract concentration, the concentration that produced the highest average diameter was the 80% concentration, but statistically it was not significantly different from the 60% concentration. This research proves the ability of thorn spinach leaves to inhibit the growth of S. mutans which causes dental caries.

Density and Distribution Patterns of Bellucia pentamera Naudin in the Rehabilitation Zone of Gunung Palung National Park, Ketapang Regency

Fiqrudina Sain Saputri — 2024-03-26

Jambu tangkalak is a tree-shaped invasive alien plant belonging to the Melastomataceae family. Jambu tangkalak (Bellucia pentamera) affects forests that are being rehabilitated and must be quickly controlled because it inhibits the growth of other plants. This study aimed to determine the density and distribution pattern of B. pentamera in the Rehabilitation Zone of Gunung Palung National Park. The data were collected from May to June 2023 using a survey method with single plot measuring 100 x 100 m in which there were sub-plots measuring 10 x 10 m. The data were analyzed by using density equations and standardized Morisita Index distribution patterns. The density of B. pentamera seedling stratum was 1485 individuals/ha with a relative density of 42.2%, sapling stratum was 2017 individuals/ha with a relative density of 57.4%, and tree stratum was 14 individuals/ha with a relative density of 0.4%. The standard value of the degree of morisita of B. pentamera nown to be 0,52. Based on this, the distribution pattern of B. pentamera is clustered with the density at the sapling stratum being more dominant than the seedling and tree strata.